Synthesis of Quantum Dots Labeled Short Peptides and Imaging the T cell Surface Receptors with QDs-Labeled Peptides
- 174 Downloads
Semiconductor quantum dots have been used for labeling many biomacromolecules and small molecules, but it remains a challenge to couple it with short active peptides that play critical roles in many physiological processes. Several binding methods for QDs and short peptides have been reported, but all with some limitations in amino acid sequence. In this paper, we report a method for synthesis of quantum dots labeled short peptides that is appropriate to any short peptide. The quantum dots (CdTe)-labeled short peptides were verified and characterized by RP-HPLC. The QDs-labeled peptides were applied to monitor the specific binding between two immune peptides and T cell surface receptors. The quantum dots-labeled immune peptides provide a powerful method for studying immunological functions of these peptides, and an effective strategy for monitoring their complex modulating processes in vivo.
KeywordsCell labeling immune peptide quantum dots
This work was supported by a grant from the Special Funds for Major State Basic Research Projects (No. 2002CB613401).
- Chan, W. and White, P.: 2000, Fmoc Solid Phase Peptide Synthesis. Oxford University PressGoogle Scholar
- Gattas-Asfura, K. M. and Leblanc, R. M.: 2003, Chem. Commun. 2684–2685Google Scholar
- Zhang, C. Y., Ma, H., Ding, Y., Jin, L., Chen, D. Y. and Nie, S.: 2000, Analyst 125(6), 1029–1031Google Scholar
- Zhang, H., Wang, L. P., Xiong, H. M., Hu, L. H., Yang, B., Li, W.: 2003a, Adv. Mater. 15(20), 1712–1715Google Scholar