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Evaluation of Biotin-OSu and Biotin-ONp in the Solid Phase Biotinylation of Peptides

  • Bodo Baumeister
  • J. Beythien
  • J. Ryf
  • P. Schneeberger
  • Peter D. White
Article

Biotinyl-oxysuccinimide and biotin p-nitrophenyl ester were evaluated in the solid phase synthesis of biotinylated peptides. Biotin p-nitrophenyl ester was found to be superior in terms of solubility and reactivity.

Keywords

Biotin Biotin N-hydroxysuccinimide ester Biotin p-nitrophenyl ester Biotinylation Fmoc solid phase peptide synthesis N-(biotinyloxy)succinimide 

Abbreviations

ACN

acetonitrile

tBu

tert-butyl

DCM

dichloromethane

DMF

dimethylformamide

Fmoc

fluoren-9-ylmethoxycarbonyl

HMP

4-hydroxymethylphenoxy

HOBt

N-hydroxybenzotriazole

RP-HPLC

reverse phase high performance liquid chromatography

Me

methyl

NMP

N-methylpyrrolidinone

ONp

p-nitrophenoxy

SPPS

solid phase peptide synthesis

TFA

trifluoroacetic acid

TIPS

triisopropylsilane

TNBS

2,4,6-trinitrobenzene sulfonic acid

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References

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Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Bodo Baumeister
    • 1
    • 3
  • J. Beythien
    • 1
  • J. Ryf
    • 1
  • P. Schneeberger
    • 1
  • Peter D. White
    • 2
  1. 1.Novabiochem, Merck Biosciences AGLäufelfingenSwitzerland
  2. 2.Novabiochem, Merck Biosciences LtdBeestonEngland
  3. 3.Correspondence Should be addressed to: Bodo Baumeister, Novabiochem, Merck Biosciences LtdBeestonEngland

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