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mRNA expression characteristics are different in irreversibly atrophic intrinsic muscles of the forepaw compared with reversibly atrophic biceps in a rat model of obstetric brachial plexus palsy (OBPP)

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Abstract

In obstetric brachial plexus palsy (OBPP), irreversible muscle atrophy occurs much faster in intrinsic muscles of the hand than in the biceps. To elucidate the mechanisms involved, mRNA expression profiles of denervated intrinsic muscles of the forepaw (IMF) and denervated biceps were determined by microarray using the rat model of OBPP where atrophy of IMF is irreversible while atrophy of biceps is reversible. Relative to contralateral control, 446 dysregulated mRNAs were detected in denervated IMF and mapped to 51 KEGG pathways, and 830 dysregulated mRNAs were detected in denervated biceps and mapped to 52 KEGG pathways. In denervated IMF, 10 of the pathways were related to muscle regulation; six with down-regulated and one with up-regulated mRNAs. The remaining three pathways had both up- and down-regulated mRNAs. In denervated biceps, 13 of the pathways were related to muscle regulation, six with up-regulated and seven with down-regulated mRNAs. Five of the pathways with up-regulated mRNAs were related to regrowth and differentiation of muscle cells. Among the 23 pathways with dysregulated mRNAs, 13 were involved in regulation of neuromuscular junctions. Our results demonstrated that mRNAs expression characteristics in irreversibly atrophic denervated IMF were different from those in reversibly atrophic denervated biceps; dysregulated mRNAs in IMF were associated with inactive pathways of muscle regulation, and in biceps they were associated with active pathways of regrowth and differentiation. Lack of self-repair potential in IMF may be a major reason why atrophy of IMF becomes irreversible much faster than atrophy of biceps after denervation.

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Acknowledgments

This research project was funded by the Chinese 973 Project Planning (2014CB542203).

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Authors and Affiliations

  1. Department of Hand Surgery, Huashan Hospital and Institutes of Biomedical Sciences, Fudan University, Shanghai, 200040, China

    Ji-xin Wu, Liang Chen & Yu-dong Gu

  2. Shanghai Key Laboratory of Peripheral Nerve and Microsurgery, Shanghai, 200040, China

    Ji-xin Wu, Liang Chen & Yu-dong Gu

  3. Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, 226001, China

    Fei Ding

  4. Department of Plastic and Peripheral Vascular Surgery, Guangdong General Hospital and Guangdong Academy of Medical Science, Guangzhou, 510080, China

    Le-zi Chen

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  1. Ji-xin Wu
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  2. Liang Chen
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  4. Le-zi Chen
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  5. Yu-dong Gu
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Correspondence to Liang Chen or Fei Ding.

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Wu, Jx., Chen, L., Ding, F. et al. mRNA expression characteristics are different in irreversibly atrophic intrinsic muscles of the forepaw compared with reversibly atrophic biceps in a rat model of obstetric brachial plexus palsy (OBPP). J Muscle Res Cell Motil 37, 17–25 (2016). https://doi.org/10.1007/s10974-016-9442-8

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  • DOI: https://doi.org/10.1007/s10974-016-9442-8

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