CXXC5 regulates differentiation of C2C12 myoblasts into myocytes
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CXXC5 is a member of the CXXC-type zinc-finger domain containing protein family, which is suggested to function in gene transcription, cell adhesion and cytoskeleton organization. Previous studies have revealed that CXXC5 is expressed in skeletal muscle, but whether it regulates skeletal myogenesis is yet unknown. Here, we screened for the possible signaling pathways in which CXXC5 might participate using luciferase gene reporters. The results indicated that CXXC5 significantly increased the activities of the promoters of genes involved in skeletal muscle differentiation. We therefore studied the role of CXXC5 during skeletal myogenesis in C2C12 myoblasts. Our findings suggest that overexpression of CXXC5 in C2C12 myoblasts facilitated myocyte differentiation, while RNAi interference of CXXC5 significantly inhibited the differentiation of C2C12 myoblasts. This study suggests that CXXC5 plays a significant role in regulating skeletal myogenesis.
KeywordsC2C12 Skeletal myogenesis Myotube Luciferase reporters Differentiation
We thank all the members of the Center for Heart Development in Hunan Normal University for their assistance. This study was supported by National Natural Science Foundation of China (30930054, 81170229, 31171402, 81170088, 31172044, 31071999, 30970425, 31272396, 81270156, 81270291,81370451,81400304,31472060, 81470377, 81470449), Hunan Provincial Natural Science Foundation of China (12JJ3117, 2015JJ3087), Hunan Provincial Innovation Foundation For Postgraduate (CX2014B200, CX2012B213), the Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province 2013-448-6, and the Scientific Research Fund of Hunan Provincial Education Department (No. 14A093).
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