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Clarification in relation to the review paper “Crossbridge and filament compliance in muscle: implications for tension generation and lever arm swing”

  • Vincenzo Lombardi
Letter to the Editor

Keywords

Actin Filament Tension Generation Combine Approach Isometric Contraction Myosin Head 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

In our experiments on single muscle fibres from Rana temporaria and Rana esculenta, using a combined approach of fast mechanics and X-ray diffraction we find that the stiffness of the myosin motor is ~3 pN/nm in both species and that the 1.5 times larger force per thick filament developed by Rana temporaria in isometric contraction is due to a correspondingly larger fraction of myosin heads attached to the actin filament (~0.3 in Rana temporaria versus ~0.2 in Rana esculenta, see for instance Decostre et al. (2005) and Piazzesi et al. (2007). In their review in J. Muscle Res. Cell Motil., Offer and Ranatunga (2010) make a contradictory interpretation of our work, and claim that the myosin motor stiffness in Rana esculenta is only 60% of that in Rana temporaria. This conclusion derives solely from their unsupported assumption that in both species the fraction of myosin heads attached to actin in isometric contraction is 0.5. We request the publication of this clarification to avoid any misleading interpretation of our work.

References

  1. Decostre V et al (2005) Effect of temperature on the working stroke of muscle myosin. Proc Natl Acad Sci USA 102:13927–13932PubMedCrossRefGoogle Scholar
  2. Offer G, Ranatunga KW (2010) Cross bridge and filament compliance in muscle: implications for tension generation and lever arm swing. J Muscle Res Cell Motil 31:245–265PubMedCrossRefGoogle Scholar
  3. Piazzesi G et al (2007) Skeletal muscle performance determined by modulation of number of myosin motors rather than motor force or stroke size. Cell 131:784–795PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.Laboratory of Physiology, DEBUniversità di FirenzeSesto FiorentinoItaly

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