Characterization of secophalloidin-induced force loss in cardiac myofibrils

  • Anna E. Bukatina
  • Gary C. Sieck
  • Kenneth B. Campbell
  • Marek Belohlavek
Original Paper


Secophalloidin (SPH) is known to cause in cardiac myofibrils force without Ca2+ (half-maximal effect ~2 mM) followed by irreversible loss of Ca2+-activated force. At maximal Ca2+ activation, SPH increases force (half-maximal effect < 0.1 mM). We found that SPH at low concentration (0.5 mM) did not cause either force activation or force loss at pCa 8.7, but both of these effects did occur when force was activated by Ca2+. The force loss was prevented when SPH was applied during rigor or in the presence of 2,3-butanedione monoxime (85 mM). Furthermore, studying muscle in which the force was previously reduced by SPH (up to 50%) did not reveal significant changes in Ca2+ sensitivity and cooperativity of Ca2+ activation or qualitative alterations in SPH-induced changes in Ca2+-activated contraction. Data suggest that the force loss is mediated by cycling cross-bridges, and might reflect a reduction in force generated by individual cross-bridges.


Actomyosin Ca2+ regulation Cardiac muscle Muscle contraction Myosin Myocardium Secophalloidin 





Adenosine 5′-(β,γ-imido)triphosphate


P1,P5-di(adenosine-5′) pentaphosphate


2,3-Butanedione monoxime


Troponin C







The authors are thankful to Dr. Eileen McMahon for critical reading of the manuscript and constructive comments. This study was supported in parts by NIH grants HL34817, and HL68555, and by a grant from GE Healthcare.


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Anna E. Bukatina
    • 1
    • 5
  • Gary C. Sieck
    • 2
    • 3
  • Kenneth B. Campbell
    • 4
  • Marek Belohlavek
    • 1
  1. 1.Division of Cardiovascular DiseasesMayo Clinic College of MedicineScottsdaleUSA
  2. 2.Department of AnesthesiologyMayo Clinic College of MedicineRochesterUSA
  3. 3.Department of Physiology & Biomedical EngineeringMayo Clinic College of MedicineRochesterUSA
  4. 4.Department of Veterinary and Comparative Anatomy, Pharmacology and PhysiologyWashington State UniversityPullmanUSA
  5. 5.ScottsdaleUSA

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