Journal of Muscle Research & Cell Motility

, Volume 26, Issue 6–8, pp 449–454 | Cite as

Molecular and biochemical characterization of kettin in Caenorhabditis elegans



Kettin is a unique member of the connectin/titin family of muscle elastic proteins, which has repetitive immunoglobulin-like domains that are separated by weakly conserved linker sequences. In striated muscles of insects and crayfish, kettin binds to actin filaments and localizes to the Z-disc and its adjacent region in the I-band. Recent sequence analysis of invertebrate connectin/titin (also known as SLS proteins) has revealed that kettin is a splice variant of connectin/titin. In contrast, in the nematode Caenorhabditis elegans, the kettin gene is independent of the genes for other connectin/titin-related proteins. Immunofluorescent localization of kettin shows that it localizes to the I-bands in the obliquely striated body wall muscle. Therefore, C. elegans is an attractive model system to study specific functions of kettin in muscle cells.


Caenorhabditis Elegans Nematode Caenorhabditis Elegans Body Wall Muscle Recent Sequence Analysis Entire cDNA Sequence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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This work is dedicated to the memory of Professor Koscak Maruyama. We are grateful to Pamela Hoppe and Robert Waterston for their gifts of the monoclonal antibodies, MH24, MH42 and MH44, Henry Epstein for his gift of the monoclonal antibody 5.6, and Laurent Segalat for his gift of the dys-1 null mutant. Wild-type C. elegans strain N2 was provided by the Caenorhabditis Genetics Center, which is funded by the National Institute of Health National Center for Research Resources. This work was supported by a grant from the National Institute of Health (R01 AR48615) to S. O.


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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  1. 1.Department of PathologyEmory UniversityAtlantaUSA
  2. 2.Department of Biophysics, Graduate School of ScienceKyoto UniversityKyoto-shiJapan

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