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Biomolecular interactions of selected buffers with hemoglobin

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Abstract

To elucidate the influence of commonly used biological buffers on the hemoglobin (Hb) structure, biomolecular interactions between Hb and the selected buffers, including tris (hydroxymethyl) aminomethane (TRIS), N-[tris (hydroxymethyl) methyl]-3-aminopropanesulfonic acid (TAPS) and N-[tris (hydroxymethyl) methyl]-2-aminoethane-sulfonic-acid (TES), are investigated by using various biophysical spectroscopic and other techniques. The techniques used in this study are ultraviolet–visible (UV–Vis), fluorescence, circular dichroism (CD) and Fourier transform infrared spectroscopy and dynamic light scattering. Fluorescence spectra analysis reveals that the addition of biological buffers increases the hydrophobicity around the tryptophan environment in Hb. Evidently, the alpha-helix structure of Hb was slightly destroyed at higher concentrations of the buffers detected by CD spectroscopy. However, the thermal stability of the protein transition temperature (Tm) gradually increases with an increase in the concentration of the biological buffers. The results also show that generally the biological buffers are able to enhance the stability of Hb.

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Acknowledgements

The authors are grateful for financing provided by the Ministry of Science and Technology (MOST), Taiwan, through Grant MOST 105-2221-E-011-144-MY3, and the international student scholarship from NTUST.

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Authors and Affiliations

  1. Department of Chemical Engineering, National Taiwan University of Science and Technology, 43 Keelung Road, Section 4, Taipei, 10607, Taiwan

    Pavani Pannuru, Bhupender S. Gupta & Ming-Jer Lee

  2. Department of Chemistry, National Tsing Hua University, 101, Section 2, Kuang-Fu Road, Hsinchu, 30013, Taiwan, R. O. C.

    Jia-Cherng Horng

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  1. Pavani Pannuru
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  2. Bhupender S. Gupta
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by PP and BSG. The first draft of the manuscript was written by PP and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Ming-Jer Lee.

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Pannuru, P., Gupta, B.S., Horng, JC. et al. Biomolecular interactions of selected buffers with hemoglobin. J Therm Anal Calorim 142, 2003–2013 (2020). https://doi.org/10.1007/s10973-020-09947-7

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