Advances in thermoanalytical techniques
- 26 Downloads
Thermogravimetry coupled with chemometrics has proved to be a rapid and cost-effective diagnostic tool for β-thalassemia screening. This model, consisting of Partial Least Square-Discriminant Analysis (PLS-DA), permitted the discrimination of thalassemic patients and healthy individuals, using thermogravimetric curves of blood samples. In this study, the impact of aspirin on the capability of the TGA/chemometric validated model to screen for thalassemia was investigated. Whole blood samples from patients with congenital defects and healthy individuals were analyzed by the TGA7 thermobalance (Perkin Elmer) without any pretreament, and the resulting curves were compared to those typical of patients after aspirin intake. The chemometric approach allowed a quick identification of the anemia and confirms that the proposed model was not affected by aspirin. Results permit to consider the coupling TGA/chemometrics as a promising diagnostic approach to provide a high-throughput and sensitive tool to obtain an early detection of thalassemia.
KeywordsThalassemia Screening Aspirin Thermogravimetric analysis Chemometrics
The authors sincerely thank the patients and their families for their willingness to contribute to this research.
- 2.Cao A, Galanello R. Beta-thalassemia. Gen Med. 2010;12:61–76.Google Scholar
- 15.Baigent C, Blackwell L, Collins R, Emberson J, Godwin J, Peto R, Hennekens C, Kearney P, Meade T, Patrono C, Roncaglioni MC, Zanchetti A. Aspirin in the primary and secondary prevention of vascular disease: collaborative meta-analysis of individual participant data from randomised trials. Lancet. 2009;373:1849–60.CrossRefGoogle Scholar
- 16.Eikelboom JW, Hirsh J, Spencer FA, Baglin TP, Weitz JI. Antiplatelet drugs: antithrombotic therapy and prevention of thrombosis. American College of Chest Physicians Evidence-Based Clinical Practice Guidelines, vol. 141. Chest, 9th edn.; 2012. p. e89S–119S.Google Scholar
- 18.Suwalsky M, Belmar J, Villena F, Gallardo MJ, Jemiola-Rzeminska M, Strzalka K. Acetylsalicylic acid (aspirin) and salicylic acid interaction with the human erythrocyte membrane bilayer induce in vitro changes in the morphology of erythrocytes. Arch Biochem Biophys. 2013;539:9–19.CrossRefGoogle Scholar
- 19.Elblbesy MA, Hereba ARM, Ahawki MM. Effects of aspirin on rheological properties of erythrocytes in vitro. Int J Biomed Sci. 2012;8:188–93.Google Scholar
- 32.Materazzi S, Finamore J, Risoluti R, Napoli A, D’Arienzo S. Characterization of thermally induced mechanisms by mass spectrometry-evolved gas analysis (EGA-MS): a study of divalent cobalt and zinc biomimetic complexes with N-heterocyclic dicarboxylic ligands. Int J Mass Spectrom. 2014;365–366:372–6.CrossRefGoogle Scholar
- 34.Massart DL, Vandeginste BGM, Buydens LMC, De Jong S, Lewi PL, Smeyers-Verbeke J. In: Vandeginste BGM, Rutan SC, editors. Handbook of chemometrics and qualimetrics, vol. Part B, 20B. Amsterdam: Elsevier; 1998. p. 88–103.Google Scholar