Journal of Thermal Analysis and Calorimetry

, Volume 133, Issue 1, pp 539–547 | Cite as

Investigation of fatty acid thermal transitions and stability in poultry pates enriched with vegetable components

  • Maria Marudova
  • Maria Momchilova
  • Ginka Antova
  • Zhana Petkova
  • Dinko Yordanov
  • Gabor Zsivanovits


The aim of the study was to describe the thermal characteristics of poultry pates enriched with vegetable components in relation to their chemical composition and technological process. Two poultry pates from chicken liver, chicken or turkey meat with vegetables were developed. The thermal characteristics of the raw materials and the ready pates were examined by differential scanning calorimetry; fatty acid profiles were detected by gas chromatography analysis. The study investigated the effect of such factors as heating/cooling rate and matrix effect of other components (e.g., proteins) in the raw materials and in the pates. It was observed that the cooling rate has a considerable effect on melting/crystallization temperature, enthalpy, and height of peaks in the process of pates fat crystallization, as well as peak height and enthalpy in the melting process. The first peaks formed during the crystallization were characterized by high instability, demonstrated by various peak shapes. The rapid cooling led to lowering of the melting point, assigned to the presence of unstable α crystals. The slow cooling led to mainly stable β′ crystals. The fraction of unsaturated fatty acids present in the fat was important for both crystallization rate and melting points in the raw materials and in the products as well. This effect was stronger in the pate products because of the presence of diverse components such as proteins. The results obtained could be used for the evaluation of thermal stability of pate fatty acids and further optimization of the pate thermal treatment.


DSC Fatty acid profile Poultry pate Chemical composition 



The study was supported by the Project No. HTAI129 (Formulation and Design of Food-products and Beverages for Preventive Nutrition) of Agricultural Academy of Bulgaria.


  1. 1.
    Svenstrup G, Brüggemann D, Kristensen L, Risbo J, Skibsted LH. The influence of pretreatment on pork fat crystallization. Eur J Lipid Sci Technol. 2005;107(9):607–15.CrossRefGoogle Scholar
  2. 2.
    Ghotra BS, Dyal SD, Narine SS. Lipid shortenings: a review. Food Res Int. 2002;35:1015–48.CrossRefGoogle Scholar
  3. 3.
    Boistelle R. Fundamentals of nucleation and crystal growth. In: Sato K, Garti N, editors. Crystallization and polymorphism of fats and fatty acids. New York: Marcel Dekker Inc; 1988.Google Scholar
  4. 4.
    Walstra P. Secondary nucleation in triglyceride crystallization. Progr Colloid Polym Sci. 1998;108:4–8.CrossRefGoogle Scholar
  5. 5.
    Relkin P, Ait-Taleb A, Sourdet S, et al. Thermal behavior of fat droplets as related to adsorbed milk proteins in complex food emulsions. A DSC study. J Am Oil Chem Soc. 2003;. Scholar
  6. 6.
    Shukat R, Bourgaux C, Relkin P. Crystallisation behaviour of palm oil nanoemulsions carrying vitamin E: dSC and synchrotron X-ray scattering studies. J Therm Anal Calorim. 2011;108(1):153–61.CrossRefGoogle Scholar
  7. 7.
    Kalnin D, et al. Monitoring fat crystallization in aerated food emulsions by combined DSC and time-resolved synchrotron X-ray diffraction. Food Res Int. 2002;35(10):927–34.CrossRefGoogle Scholar
  8. 8.
    Relkin P, Sourdet S, Fosseux PY. Fat crystallization in complex food emulsions: effects of adsorbed milk proteins and of a whipping process. J Therm Anal Calorim. 2003;71(1):187–95.CrossRefGoogle Scholar
  9. 9.
    International Organization for Standardization (ISO). Animal and vegetable fats and oils—Gas chromatography of fatty acid methyl esters—Part 2: Preparation of methyl esters of fatty acids. (Standard No. 12966–2:2017). 2017;
  10. 10.
    International Organization for Standardization (ISO). Animal and vegetable fats and oils—Gas chromatography of fatty acid methyl esters—Part 1: Guidelines on modern gas chromatography of fatty acid methyl esters. (Standard No. 12966–1:2014). 2014;
  11. 11.
    Litwinenko JW, Rojas AM, Gerschenson LN, Marangoni AG. Relationship between crystallization behavior, microstructure, and mechanical properties in a palm oil-based shortening. J Am Oil Chem Soc. 2002;79:647–54.CrossRefGoogle Scholar
  12. 12.
    Kang MJ, Shin MS, Park JN, Lee SS. The effects of polyunsaturated: saturated fatty acids ratios and peroxidisability index values of dietary fats on serum lipid profiles and hepatic enzyme activities in rats. Br J Nutr. 2005;94:526–32.CrossRefGoogle Scholar
  13. 13.
    deMan L, deMan JM, Blackman B. Physical and textural characteristics of some North American shortenings. J Am Oil Chem Soc. 1991;68:63–9.CrossRefGoogle Scholar
  14. 14.
    Hodge SM, Rousseau D. Flocculation and coalescence in water-in-oil emulsions stabilized by paraffin wax crystals. Food Res Int. 2003;36:695–702.CrossRefGoogle Scholar
  15. 15.
    Decca MB, Perduca M, Monaco HL, Montich GG. Conformational changes of chicken liver bile acid-binding protein bound to anionic lipid membrane are coupled to the lipid phase transitions. BBA Biomembr. 2007;1768(6):1583–91.CrossRefGoogle Scholar
  16. 16.
    Elodie A, Relkin P, Pina M, Collignan A. Characterisation of chicken fat dry fractionation at the pilot scale. Eur J Lipid Sci Technol. 2004;106(9):591–8.CrossRefGoogle Scholar
  17. 17.
    Galluzzo SJ, Regenstein JM. Role of chicken breast muscle proteins in meat emulsion formation: myosin, actin and synthetic actomyosin. J Food Sci. 1978;43(6):1761–5.CrossRefGoogle Scholar
  18. 18.
    Lopez C, Bourgaux C, Lesieur P, Bernadou S, Keller G, Ollivon M. Thermal and structural behavior of milk fat. 3. Influence of cooling rate and droplet size on cream crystallization. J Colloid Int Sci. 2002;254:64–78.Google Scholar
  19. 19.
    Vanapalli SA, Palanuwech J, Coupland JN. Stability of emulsions to dispersed phase crystallization: effect of oil type, dispersed phase volume fraction, and cooling rate. Colloids Surf A Physicochem Eng Asp. 2002;204:227–37.CrossRefGoogle Scholar
  20. 20.
    Walstra P. Crystallization. In: Walstra P, editor. Physical chemistry of foods. New York: Marcel Dekker Inc; 2003. p. 583–649.Google Scholar

Copyright information

© Akadémiai Kiadó, Budapest, Hungary 2017

Authors and Affiliations

  1. 1.Department of Physics, Faculty of Physics and TechnologyUniversity of Plovdiv Paisii HilendarskiPlovdivBulgaria
  2. 2.Food Research and Development Institute (FoodRDI)PlovdivBulgaria
  3. 3.Department of Chemical Technology, Faculty of ChemistryUniversity of Plovdiv Paisii HilendarskiPlovdivBulgaria
  4. 4.Department of Meat and Fish TechnologyUniversity of Food TechnologyPlovdivBulgaria

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