Skip to main content
SpringerLink
Log in

Differential scanning calorimetry (DSC) of blood serum in chronic obstructive pulmonary disease (COPD)

A new diagnostic tool ahead?

  • Published:
Journal of Thermal Analysis and Calorimetry Aims and scope Submit manuscript

Abstract

Chronic obstructive pulmonary disease (COPD) is a major global health challenge with a gloom perspective of being one of the big three cause of death by 2020. No reliable/reproducible biomarker has been identified so far to match the clinically-based staging system (GOLD). Blood samples of 30 subjects divided into 6 groups (no-COPD/-smoker, no-COPD/non-smoker, COPD I, COPD II, COPD III, COPD IV) with 5 patients in each were tested by differential scanning calorimetry. There is a clear 15.4 % difference between the heat flow maxima measured when no-COPD subjects were compared in accordance to their smoking/non-smoking status. Odds ratio of different heat flow in actively smoking COPD patients in stage IV and stage I was 1.61. A reverse tendency is detected in the relevant non-smoking COPD groups. The differences are inconsistent in intermediate stages (COPD II and III). DSC seems to be an applicable and objective method for monitoring nicotine abuse. There is a chance to detect specific typology of thermokinetic patterns in the two extremes of COPD (I vs. IV). Further studies with increased sample size are needed to allow calculations on specificity/sensitivity/positive and negative predictive value of enthalpies and heat flow maximums. The first clinically relevant blood-based COPD marker on the intravascular side of the alveo-capillary screen is demonstrated by our pilot study.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Rabe KF, Hurd S, Anzueto A, Barnes PJ, Buist SA, Calverly P, Fukuchi Y, Jenkins C, Rodriquez-Roisin R, van Weel C, Zielinski J. Global strategy for the diagnosis, management, and prevention of chronic obstructive pulmonary disease: GOLD executive summary. Am J Resp Crit Care Med. 2007;176:532–55.

    Article  Google Scholar 

  2. Buist AS. Study on the burden on patients with chronic obstructive pulmonary disease. Proc Am Thorac Soc. 2008;5(8):796–9.

    Article  Google Scholar 

  3. Sin DD, McAlister FA, Man SFP, Anthonisen NR. Contemporary management of chronic obstructive pulmonary disease: scientific review. JAMA. 2003;290(17):2301–12.

    Article  CAS  Google Scholar 

  4. Agusti A, Sobradillo P, Celli B. Addressing the complexity of chronic obstructive pulmonary disease: from phenotypes and biomarkers to scale-free networks, systems biology, and P4 medicine. Am J Respir Crit Care Med. 2011;183:1129–37.

    Article  Google Scholar 

  5. Turino GM. COPD and biomarkers: the search goes on. Thorax. 2008;63:1032–4.

    Article  Google Scholar 

  6. Sin DD, Man FP. Biomarkers in COPD. Are we there yet? Chest. 2008;133(6):1296–8.

    Article  Google Scholar 

  7. Bucher HC, Guyatt GH, Cook DJ, Holbrook A, McAlister FA. Users’ guides to the medical literature: XIX. Applying clinical trial results: A. How to use an article measuring the effect of an intervention on surrogate end points: Evidence-Based Medicine Working Group. JAMA. 1999;282:771–8.

    Article  CAS  Google Scholar 

  8. Cazzola M, MacNee W, Martinez FJ, Rabe KF, Franciosi LG, Barnes PJ, Brusasco V, Burge P, Calverley PMA, Celli BR, Jones PW, Mahler DA, Make B, Miravitlles M, Page CP, Palange P, Parr D, Pistolesi M, Rennard SI, Rutten-van Mölken MP, Stockley R, Sullivan SD, Wedzicha JA, Wouters EF, On behalf of the American Thoracic Society/European Respiratory Society Task Force on outcomes of COPD. Outcomes for COPD pharmacological trials: from lung function to biomarkers. Eur Respir J. 2008;31(2):416–69.

    Google Scholar 

  9. Man SF, Connett JE, Anthonisen NR, Wise RA, Tashkin DP, Sin DD. C-reactive protein and mortality in mild to moderate chronic obstructive pulmonary disease. Thorax. 2006;61:849–53.

    Article  CAS  Google Scholar 

  10. Pinto-Plata V, Toso J, Lee K, Park D, Bilello J, Mullerova H, De Souz MM, Vessey RS, Celli BR. Profiling serum biomarkers in patients with COPD: associations with clinical parameters. Thorax. 2007;62:595–601.

    Article  Google Scholar 

  11. Hurst JR, Donaldson GC, Perera WR, Wilkinson TMA, Bilello JA, Hagan GW, Vessey RS, Wedzicha JA. Use of plasma biomarkers at exacerbation of chronic obstructive pulmonary disease. Am J Respir Crit Care Med. 2006;17:867–74.

    Article  Google Scholar 

  12. Dahl M, Vestbo J, Lange P, Bojesen SE, Tybjaerg-Hansen A, Nordestgaard BG. C-reactive protein as a predictor of prognosis in chronic obstructive pulmonary disease. Am J Respir Crit Care Med. 2007;175:250–5.

    Article  CAS  Google Scholar 

  13. Aronson D, Roterman I, Yigla M, Kerner A, Avizohar O, Sella R, Bartha P, Levy Y, Markiewicz W. Inverse association between pulmonary function and C-reactive protein in apparently healthy subjects. Am J Respir Crit Care Med. 2006;174:626–32.

    Article  CAS  Google Scholar 

  14. De Torres JP, Cordoba-Lanus E, Lopez-Aguilar C, Muros de Fuentes M, Montejo de Garcini A, Aquirre-Jaime A, Celli BR, Casanova C. C-reactive protein levels and clinically important predictive outcomes in stable COPD patients. Eur Respir J. 2006;27:902–7.

    Google Scholar 

  15. De Torres JP, Pinto-Plata V, Casanova C, Mullerova H, Córdoba-Lanús E, Muros de Duentes M, Aquirre-Jaime A, Celli BR. C-reactive protein levels and survival in patients with moderate to very severe COPD. Chest. 2008;133:1336–43.

    Article  Google Scholar 

  16. Nissen SE, Tuzcu EM, Schoenhagen P, Tuzcu M, Schoenhagen P, Crowe T, Sasiela WJ, Tsai J, Orazem J, Magorian RD, O’Shaughnessy C, Ganz P. Statin therapy, LDL cholesterol, C-reactive protein, and coronary artery disease. N Engl J Med. 2005;352:29–38.

    Article  CAS  Google Scholar 

  17. Pepys MB, Hirschfield GM. C-reactive protein: a critical update. J Clin Invest. 2003;111:1805–12.

    CAS  Google Scholar 

  18. Wang TS, Duan SF, Tang TQ. Hemorrheologic changes in COPD patients with or without cor pulmonale. J Tongji Med Univ. 1998;8(4):235–7.

    Google Scholar 

  19. Coppola L, Verrazzo G, Esposito G, La Marca C, Mazzarelli R, Guastafierro S, Giunta R, Giugliano D, Varricchio M. Hemorheological and cardiovascular effects of exercise training in the rehabilitation of elderly patients with chronic obstructive pulmonary disease. Arch Gerontol Geriatr. 1999;28(1):1–8.

    Article  CAS  Google Scholar 

  20. Michnik A, Michalik K, Kluczewska A, Drzazga Z, Comparative DSC. study of human and bovine serum albumin. J Therm Anal Calorim. 2006;84:113–7.

    Article  CAS  Google Scholar 

  21. Domán I, Illés T, Lőrinczy D. Differential scanning calorimetric examination of the human intervertebral disc: establishment of calorimetric standards of different stage sof degeneration. Thermochim Acta. 2003;405(2):293–9.

    Article  Google Scholar 

  22. Than P, Domán I, Lőrinczy D. Differential scanning calorimetry in the research of degenerative musculoskeletal disorders. Thermochim Acta. 2004;415(1–2):83–7.

    Article  CAS  Google Scholar 

  23. Szántó Z, Kovács GY, Nagy V, Rőth E, Molnar TF, Horváth OP. Differential scanning calorimetric examination of the tracheal cartilage after primary reconstruction with different suturing techniques. Thermochim Acta. 2006;445:190–4.

    Article  Google Scholar 

  24. Michnik A, Drzazga Z. Thermal denaturation of mixtures of human serum proteins. J Therm Anal Calorim. 2010;101:513–8.

    Article  CAS  Google Scholar 

  25. Michnik A, Drzazga Z, Michalik K, Barczyk A, Santura I, Sozan′ska E, Pierzchała W. Differential scanning calorimetry study of blood serum in chronic obstructive pulmonary disease. J Therm Anal Calorim. 2010;102:57–60.

    Google Scholar 

  26. Sharma D. Non-isothermal unfolding/denaturing kinetics of egg white protein. J Therm Anal Calorim. 2012;109:1139–43.

    Article  CAS  Google Scholar 

  27. Cholewka A, Knefel G, Stanek A, Kawecki M, Nowak M, Sieroń A, Drzazga Z. Thermal imaging and TC oximetry measurements of hyperbaric oxygen therapy (HBO) effects on trophic ulceration of the crura. J Therm Anal Calorim. 2012;108:25–31.

    Article  Google Scholar 

  28. Saleh SW, Moreno-Molek SE, Mantheni DR, Maheswaram MPK, Sam-Yellowe T, Riga AT. Thermal behavior and signature patterns of human cytokine and soluble cytokine receptors investigated using dielectric thermal analysis and thermogravimetry. J Therm Anal Calorim. 2012;108:41–51.

    Article  CAS  Google Scholar 

  29. Read J. The lungs and circulation in chronic pulmonary disease. J R Coll Physicians Lond. 1971;5(3):221–31.

    CAS  Google Scholar 

  30. Schönhofer B. The optimal hematocrit and hemoglobin values in lung diseases. Wien Klin Wochenschr. 2001;113(Suppl 1):6–8.

    Google Scholar 

  31. Boussuges A, Rossi P, Gouitaa M, Nussbaum E. Alterations in the peripheral circulation in COPD patients. Clin Physiol Funct Imaging. 2007;27(5):284–90.

    Article  Google Scholar 

  32. Kuebler WM. Selectins revisited: the emerging role of platelets in inflammatory lung disease. J Clin Invest. 2006;116(12):3106–8.

    Article  CAS  Google Scholar 

  33. Similowski T, Agustí A, MacNee W, Schönhofer B. The potential impact of anaemia of chronic disease in COPD. Eur Respir J. 2006;27(2):390–6. (Review. Erratum in Eur Respir J. 2006;27(5):1076).

    Article  CAS  Google Scholar 

  34. Gale NS, Duckers JM, Enright S, Cockcroft JR, Shale DJ, Bolton CE. Does pulmonary rehabilitation address cardiovascular risk factors in patients with COPD? BMC Pulm Med. 2011;11:20.

    Article  Google Scholar 

Download references

Acknowledgements

The SETARAM Micro DSC-II was purchased with a grant (CO-272) from the Hungarian Scientific Research Fund (D. Lőrinczy).

Author information

Authors and Affiliations

  1. Department of Pulmonology, Petz Aladár Hospital, Gyor, Hungary

    Zsuzsanna Szalai

  2. Department of Pulmonology, Karolina Hospital, Régi Vámház ter 2-4, Mosonmagyaróvár, 9200, Hungary

    Zsuzsanna Szalai

  3. Thoracic Surgery Unit, Department of Surgery, Petz Aladár Teaching Hospital, Vasvári Pál 2-4, Gyor, 9023, Hungary

    Tamás F. Molnár

  4. Faculty of Medicine, Institute of Biophysics, University of Pécs, Szigeti str. 12, Pécs, 7624, Hungary

    Dénes Lőrinczy

Authors
  1. Zsuzsanna Szalai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tamás F. Molnár
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dénes Lőrinczy
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Dénes Lőrinczy.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Szalai, Z., Molnár, T.F. & Lőrinczy, D. Differential scanning calorimetry (DSC) of blood serum in chronic obstructive pulmonary disease (COPD). J Therm Anal Calorim 113, 259–264 (2013). https://doi.org/10.1007/s10973-013-2999-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10973-013-2999-1

Keywords

Search

Navigation