Abstract
Splenic lymphocytes play an important role in host acute or chronic diseases. The abnormality of these cells in the spleens of humans might lead to some riskful diseases for human. Hence, in this study, the effects of two ginsenosides Rg1 and Rb1 on splenic lymphocytes growth were studied by microcalorimetry. Some qualitative and quantitative information, such as the metabolic power-time curves, growth rate constant k, maximum heat-output power of the exponential phase P max, total heat output Q t of splenic lymphocytes were obtained to present the effects of Rg1 and Rb1 on these cells. The values of k, P max, and Q t from the thermogenic growth curves of splenic lymphocytes were found to increase in the presence of Rg1, while the change was adverse for Rb1, illustrating that Rg1 had promotion effect and Rb1 had inhibitory effect on splenic lymphocytes growth and these promotion or inhibitory effects were enhanced with increasing the concentration of the two compounds, respectively. The microcalorimetric results were confirmed by MTT assay for determining the MTT optical density (OD) value and [3H] Thymidine incorporation assay ([3H]-TdR) for determining the count per minute (cpm) value: Rg1 could increase the MTT OD value and the cpm value of [3H]-TdR incorporation into splenic lymphocytes, and these values were increased with increasing the concentration of this compound, while Rb1 had the adverse results. The structure–activity relationships showed that the glucopyranoside and hydroxyl groups at the dammarane-type mother nucleus skeleton might play a crucial role for the opposing effects of the two ginsenosides on splenic lymphocytes. Compared with the other two assay methods, the microcalorimetric method provided more useful and reliable information for quickly and objectively evaluating the effects of drugs or compounds on the living cells, which would be a highly promising analytical tool for the characterization of the biological process and the estimation of the drugs’ efficiency.
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References
Nah SY, Kim DH, Rhim H. Ginsenosides: are any of them candidates for drugs acting on the central nervous system? CNS Drug Rev. 2007;13:381–404.
Nah SY, Bhatia KS, Lyles J, Ellinwood EH, Lee TH. Effects of ginseng saponin on acute cocaine-induced alterations in evoked dopamine release and uptake in rat brain nucleus accumbens. Brain Res. 2009;1248:184–90.
Chen CF, Chiou WF, Zhang JT. Comparison of the pharmacological effects of Panax ginseng and Panax quinquefolium. Acta Pharmacol Sinica. 2008;29:1103–8.
Shibata S, Tanaka O, Shoji J, Saito H. Chemistry and pharmacology of Panax. In: Wagner H, Hikino H, Farnsworth NR, editors. Economic and medicinal plant research, vol. 1. London, Orlando, SanDiego, New York, Toronto, Montreal, Sydney, Tokyo: Academic Press; 1985. p. 217–87.
Sengupta S, Toh SA, Sellers LA, Skepper JN, Koolwijk P, Leung HW, Yeung HW, Wong RN, Sasisekharan R, Fan TP. Modulating angiogenesis: the yin and the yang in ginseng. Circulation. 2004;110:1219–25.
Panayi GS, Lanchbury JS, Kingsley GH. The importance of the T-cell in initiating and maintaining the chronic synovitis of rheumatoid arthritis. Arth Rheumatol. 1992;35:729–35.
Murphy KM. T lymphocyte differentiation in the periphery. Curr Opin Immunol. 1998;10:226–32.
Kong WJ, Wang JB, Jin C, Zhao YL, Dai CM, Xiao XH, Li ZL. Effect of emodin on Candida albicans growth investigated by microcalorimetry combined with chemometric analysis. Appl Microbiol Biotechnol. 2009;83:1183–90.
Kong WJ, Jin C, Xiao XH, Zhao YL, Li ZL, Zhang P, Liu W, Li XF. Comparative study of effects of two bile acid derivatives on Staphylococcus aureus by multiple analytical methods. J Hazard Mater. 2010;179:742–7.
Wadsö I. Characterization of microbial activity in soil by use of isothermal microcalorimetry. J Therm Anal Calorim. 2009;95:843–50.
Afzal AB, Akhtar MJ, Svensson LG. Thermal studies of DBSA-doped polyaniline/PVC blends by isothermal microcalorimetry. J Therm Anal Calorim. 2010;100:1017–25.
Zhao H, Bennici S, Shen J, Auroux A. Calorimetric study of the acidic character of V2O5–TiO2/SO4 2− catalysts used in methanol oxidation to dimethoxymethane. J Therm Anal Calorim. 2010;99:843–7.
Zhao YL, Yan D, Wang JB, Zhang P, Xiao XH. Anti-fungal effect of berberine on Candida albicans by microcalorimetry with correspondence analysis. J Therm Anal Calorim. doi:10.1007/s10973-009-0565-7.
Kong WJ, Li ZL, Xiao XH, Zhao YL, Zhang P. Activity of berberine on Shigella dysenteriae investigated by microcalorimetry and multivariate analysis. J Therm Anal Calorim. doi:10.1007/s10973-010-0778-9.
Yang LN, Sun LX, Xu F, Zhang J, Zhao JN, Zhao ZB, Song CG, Wu RH, Ozao R. Inhibitory study of two cephalosporins on E. coli by microcalorimetry. J Therm Anal Calorim. 2010;100:589–92.
Li X, Zhang T, Min XM, Liu P. Toxicity of aromatic compounds to Tetrahymena estimated by microcalorimetry and QSAR. Aquat Toxicol. 2010;98:322–7.
Kong WJ, Zhao YL, Xiao XH, Li ZL, Jin C, Li HB. Investigation of the anti-fungal activity of coptisine on Candida albicans growth by microcalorimetry combined with principal component analysis. J Appl Microbiol. 2009;107:1072–80.
Zhou PJ, Zhou HT, Yi P, Liu Y, Wu ZB, Qu SS, Zhu YG. Microcalorimetric studies on the thermogenesis of energy release of mitochondria isolated from rice. Microchem J. 2001;69:103–9.
Tan AM, Xu B, Huang SQ, Qu SS. Thermochemical study on the growth metabolism of human promyelocytic leukemia HL-60 cells inhibited by water-soluble metalloporphyrins. Thermochim Acta. 1999;333:99–102.
Liu M, Zhang JT. Studies on the mechanisms of immunoregulatory effects of ginsenoside Rg1 in aged rats. Acta Pharm Sin. 1996;31:95–100.
Liu D, Li B, Liu Y, Attele AS, Kyle JW, Yuan CS. Voltage-dependent inhibition of brain Na(+) channels by American ginseng. Eur J Pharmacol. 2001;413:47–54.
Zhang GJ. Bioassay on traditional Chinese medicine. Beijing: China Chemical Industry Press; 2006.
Xiao XH, Jin C, Zhao ZZ, Xiao PG, Wang YY. Probe into innovation and development of pattern of quality control and evaluation for Chinese medicine. China J Chin Mater Med. 2007;32:1377–81.
Xiao XH, Yan D, Wang JB, Jin C. Some opinions on bioassay for the quality control of Chinese medicines. World Sci Tech/Mod Tradit Chin Med Mater Medica. 2009;11:504–8.
Braissant O, Wirz D, Göpfert B, Daniels AU. Use of isothermal microcalorimetry to monitor microbial activities. FEMS Microbiol Lett. 2010;33:1–8.
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This study was supported by Foundation of National Basic Research Program of China (No. 2007CB512607) and National Nature Science Foundation (No. 30772740).
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Zhao, YL., Wang, JB., Zhang, P. et al. Microcalorimetric study of the opposing effects of ginsenosides Rg1 and Rb1 on the growth of mice splenic lymphocytes. J Therm Anal Calorim 104, 357–363 (2011). https://doi.org/10.1007/s10973-010-1003-6
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DOI: https://doi.org/10.1007/s10973-010-1003-6