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Thermal denaturation of mixtures of human serum proteins

DSC study

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Abstract

Differential scanning calorimetry (DSC) has been employed to study the thermal denaturation processes of the main protein fractions of blood serum. These processes have been compared for albumins (nondefatted (HSA) and fatty acid free (HSAf)), α,β-globulins, γ-globulins, and their mixtures in aqueous (pH 6.5) and buffer (pH 7.2) solutions. The results have indicated that α,β-globulins inhibit γ-globulins’ aggregation in buffer solutions. The decrease of stability of HSA and HSAf aqueous solutions has been observed in the presence of γ-globulins. The mixtures of albumins and γ-globulins have revealed the tendency to ready aggregation in water. Moreover, the results have suggested that neither γ-globulins nor albumins severely change the stability of α,β-globulins.

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Authors and Affiliations

  1. A. Chełkowski Institute of Physics, Department of Medical Physics, University of Silesia, ul. Uniwerytecka 4, 40-007, Katowice, Poland

    Anna Michnik & Zofia Drzazga

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  1. Anna Michnik
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  2. Zofia Drzazga
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Correspondence to Anna Michnik.

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Michnik, A., Drzazga, Z. Thermal denaturation of mixtures of human serum proteins. J Therm Anal Calorim 101, 513–518 (2010). https://doi.org/10.1007/s10973-010-0826-5

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  • DOI: https://doi.org/10.1007/s10973-010-0826-5

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