Journal of Thermal Analysis and Calorimetry

, Volume 102, Issue 1, pp 217–223 | Cite as

Study on the binding of puerarin to bovine serum albumin by isothermal titration calorimetry and spectroscopic approaches



The interaction of a flavonoid molecule (puerarin) with bovine serum albumin (BSA) was characterized by isothermal titration calorimetry (ITC), optical spectroscopic technique, and molecular modeling method under physiological conditions. The binding parameters for the reaction were calculated according to ITC experiments at different temperatures. The thermodynamic parameters, negative enthalpy changes (ΔH), and positive entropy (ΔS) indicated that the binding processes were entropically driven. The alterations of protein secondary structure in the presence of puerarin in aqueous solution were estimated by the evidences from FT-IR and CD spectroscopy with reductions of α-helices. On the basis of fluorescence resonance energy transfer (FRET) between excited tryptophan in BSA and BSA bound puerarin, the critical transfer distance and mean distance between tryptophan in BSA and puerarin were estimated.


Bovine serum albumin Puerarin Binding ITC FRET Thermodynamic parameters 


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Copyright information

© Akadémiai Kiadó, Budapest, Hungary 2010

Authors and Affiliations

  1. 1.Department of PharmacologyMedical School of Yangzhou UniversityYangzhouPeople’s Republic of China
  2. 2.School of Chemistry and Chemical EngineeringYangzhou UniversityYangzhouPeople’s Republic of China

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