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Reversible unfolding of poplar iso-plastocyanins

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Abstract

We attempted to determine the experimental conditions under which poplar plastocyanin iso-forms PCa and PCb undergo reversible thermal unfolding studied by differential scanning calorimetry (DSC). Our results indicate that an exothermic unfolding process exists always in the presence of molecular oxygen. Reversible unfolding and almost perfect two-state transitions were exhibited in the presence of TCEP under anaerobic conditions. This suggests that the second endothermic peak is due to copper-site disulfide dimmers formed during thermal denaturation. The conformational thermal stability of reduced PCb (ΔG (25 °C) = 33.9 kJ mol−1) has proven to be higher than that of reduced PCa (ΔG(25 °C) = 22.9 kJ mol−1).

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Acknowledgements

This work was supported by grants of Fund “Scientific researches” of the Bulgarian Ministry of Education and Sciences (contract No. MY-Б-1511/2005 and No. Б-1519/05/2005).

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Authors and Affiliations

  1. Bulgarian Academy of Sciences, Institute of Biophysics, Acad.G.Bonchevstr. bl.21, 1113, Sofia, Bulgaria

    V. I. Getov, G. R. Toromanov, G. K. Kostov, M. I. Dimitrov & A. Ch. Shosheva

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  1. V. I. Getov
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  2. G. R. Toromanov
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  3. G. K. Kostov
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  4. M. I. Dimitrov
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  5. A. Ch. Shosheva
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Correspondence to V. I. Getov.

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Getov, V.I., Toromanov, G.R., Kostov, G.K. et al. Reversible unfolding of poplar iso-plastocyanins. J Therm Anal Calorim 98, 877–883 (2009). https://doi.org/10.1007/s10973-009-0141-1

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  • DOI: https://doi.org/10.1007/s10973-009-0141-1

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