Journal of Thermal Analysis and Calorimetry

, Volume 95, Issue 3, pp 769–774 | Cite as

DSC analysis of human fat tissue in steroid induced osteonecrosis

A preliminary study
  • G. Bognár
  • Cs. Pintér
  • B. Horváth
  • T. Sydo
  • E. Ligeti
  • J. Pulai
  • D. Lőrinczy
Medical Aspects


Osteonecrosis (ON) of the femoral frequently occurs after steroid medication. One of the final pathways leading to steroid induced ON is thought to be pathologic fat metabolism. The pathobiological mechanism underlying the induction of fat metabolism outslides by steroids leading to ON has not been fully elucidated. The purpose of this study was to examine the intraoperative obtained gluteal fat tissue from ON patients with histology, gas chromatography (GC) and differential scanning calorimetry (DSC) and to compare them with otherwise healthy patient’s samples. The histological sections showed no significant differences compared with the control group. GC revealed that fraction of saturated fatty acids decreased in ON samples from mean values of controls of 24% to 21, the polyunsaturated fraction from 20 to 14%. The monounsaturated acids showed an increase from mean rate of 52% of the controls to 65% of steroid treated samples. DSC curves correlate with chromatographic analysis of the tissue fatty acids (Steroid treated, heating between 0–100°C: T m=5.7°C, ΔH= −15.8J/g−1; heating between −20–100°C: Tm= −9.96 and 5.85°C, ΔH= −59.17 and −16.2 J g−1. Non-necrotic, heating between 0–100°C: two separable transition with Tm=5.7 and 9.9°C, total ΔH= −20.8 J g−1; heating between −20–100°C: Tm= −10.9 and 4.95°C, total ΔH= −75.8 J g−1.)

Our preliminary findings are rather tendentious. Further investigations are needed with higher sample rate and under other anamnestic circumstances too.


DSC fatty acids gas chromatography osteonecrosis 


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Copyright information

© Akadémiai Kiadó, Budapest, Hungary 2009

Authors and Affiliations

  • G. Bognár
    • 1
  • Cs. Pintér
    • 1
  • B. Horváth
    • 1
  • T. Sydo
    • 2
  • E. Ligeti
    • 3
  • J. Pulai
    • 4
  • D. Lőrinczy
    • 5
  1. 1.Department of Traumatology and OrthopaedicsVeszprém County HospitalVeszprémHungary
  2. 2.Department of Internal MedicineVeszprém County HospitalVeszprémHungary
  3. 3.Department of PathologyVeszprém County HospitalVeszprémHungary
  4. 4.Department of RheumatologyVeszprém County HospitalVeszprémHungary
  5. 5.Institute of BiophysicsUniversity of Pécs, Faculty of MedicinePécsHungary

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