Effect of berberine alkaloids on Bifidobacterium adolescentis growth by microcalorimetry
- 105 Downloads
The inhibitory effects of three berberine alkaloids (BAs) from Coptis chinensis Franch on Bifidobacterium adolescentis growth were investigated by microcalorimetry. The growth rate constant (k) and maximum heat-output power (Pmax) decreased and peak time of maximum heat-output power (tp) prolonged with the increase of BAs concentration. Half inhibitory ratios (IC50) BAs were respectively 790.3 (berberine), 339.6 (coptisine) and 229.8 μL−1 (palmatine), which indicated the sequence of their antimicrobial activity: berberine<coptisine<palmatine. Combined with previous findings, the sequence which could show the bioactivity of Bacillus shigae and Escherichia coli was: berberine>coptisine>palmatine. The structure-function relationship of BAs indicated that the functional group methylenedioxy or methoxyl at C2 and C3 might be the major group inducing the activities of BAs on E. coli and B. adolescentis. Meanwhile, the substituent groups at C2, C3, C9 and C10 almost had equal effect on B. shigae.
KeywordsBacillus shigae berberine alkaloid Bifidobacterium adolescentis Escherichia coli inhibitory effect intestinal flora microcalorimetry
Unable to display preview. Download preview PDF.
- 1.China Pharmacopoeia Committee, Pharmacopoeia of the People’s Republic of China (1st Div., 2005 ed.), Beijing 2005, p. 213.Google Scholar
- 2.D. M. Ding, Pharmacodynamic Action and Clinic of Chinese Medicinal Materials, Beijing 1999, p. 154.Google Scholar
- 3.H. X. Guo, Foundation and Application of Probiotics, Beijing 2002, p. 3.Google Scholar
- 5.X. S. Shen, Y. Liu, C. P. Zhou and R. M. Zhao, Acta Chim. Sinica, 58 (2000) 1463.Google Scholar
- 19.X. Y. Su, PhD Thesis, Dalian Institute Chem. Phys., Chin. Acad. Sci., China (2006) Dalian.Google Scholar