Microcalorimetric investigation of the growth of the Escherichia coli DH5α in different antibiotics
The effects of Amoxicillin Sodium and Cefuroxime Sodium on the growth of E. coli DH5α were investigated by microcalorimetry. The metabolic power-time curves of E. coli DH5α growth were determined by using a TAM air isothermal microcalorimeter at 37°C. By evaluation of the obtained parameters, such as growth rate constants (k), inhibitory ratio (I), the maximum heat power (P m) and the time of the maximum heat power (t m), one found that the inhibitory activity of Amoxicillin Sodium vs. E. coli DH5α is enhanced with the increasing of the Amoxicillin Sodium concentration, and the Cefuroxime Sodium has a stimulatory effect on the E. coli DH5α growth when the concentration is about 1 μg mL−1. The IC50 for the Amoxicillin Sodium and the Cefuroxime Sodium are 1.6 and 2.0 μg mL−1, respectively, it implicates that the E. coli DH5α is more sensitive to Amoxicillin Sodium than Cefuroxime Sodium.
KeywordsAmoxicillin Sodium Cefuroxime Sodium E. coli DH5α inhibition microcalorimetry
Unable to display preview. Download preview PDF.
- 13.O. Culic, M. L. H. Gruwel and J. Schrader, Am. J. Physiol., 273 (1997) C205.Google Scholar
- 14.M. L. H. Gruwel, C. Alves and J. N. Schrader, Am. J. Physiol., 268 (1995) H351.Google Scholar
- 17.F. Xu, L. X. Sun, Z. C. Tan, J. G. Liang, C. G. Song and T. Zhang, 4th Internationanl and 6th Japan-China Joint Symposium on Calorimetry and Thermal Analysis, Fukuoka, Japan 2005, p. 170.Google Scholar