DSC examinations on cataractous lens materials obtained by phacoemulsification

  • Biró Zs. 
  • Nemes Vanda 
  • Molnár G. A. 
  • Wittmann I. 
  • Ludány Andrea 
  • Lőrinczy D. 


Modifications of lens proteins play a crucial role in the formation of cataract, which is among the leading causes of world blindness in the ageing population. Although modern cataract surgery by phacoemulsification is a very successful and safe procedure, the prevention of cataract formation would be a real breakthrough in this field of ophthalmology.

The aim of our study was to analyse the thermal denaturation of the cataractous lens proteins by the method of differential scanning calorimetry (DSC), to understand cataract formation and to work on its prevention possibilities. Samples were obtained from cataract patients of different age, sex, patients with and without diabetes mellitus. Samples were obtained from lenses of mature degree and progredient degree of cataract as well.

Previous DSC examinations were performed on manually extracted human cataractous lens materials, however to the best of our knowledge, this is the first study, in which DSC examinations were performed on lens materials obtained by the phacoemulsification technique, which gained acceptance world-wide in the last decade.


cataract diabetes DSC phacoemulsification 


  1. 1.
    Van Boekel, MA, Hoenders, HJ 1992FEBS Letters3141CrossRefGoogle Scholar
  2. 2.
    Fu, S, Dean, R, Southan, M, Truscott, R 1998J. Biol. Chem.27328603CrossRefGoogle Scholar
  3. 3.
    Wells-Knecht, MC, Huggins, TG, Dyer, DG, Thorpe, SR, Baynes, JW 1993J. Biol. Chem.26812348Google Scholar
  4. 4.
    Bloemendal, H, de Jong, W, Jaenicke, R, Lubsen, NH, Slingsby, C, Tardieu, A 2004Progr. Biophys. Mol. Biol.86407CrossRefGoogle Scholar
  5. 5.
    Davies, MJ, Fu, S, Wang, H, Dean, RT 1999Free Rad. Biol. Med.271151CrossRefGoogle Scholar
  6. 6.
    Siebinga, I, Vrensen, GF, Otto, K, Puppels, GJ, De Mul, FF, Greve, J 1992Exp. Eye. Res.54759CrossRefGoogle Scholar
  7. 7.
    Stadtman, ER, Levine, RL 2003Amino Acids25207CrossRefGoogle Scholar
  8. 8.
    Adler’s physiology of the eye. (Ed.: Williams M. Hart, 9th Edition) Mosby Year Book Inc., 1992, pp. 348–90.Google Scholar
  9. 9.
    Bron, AJ, Vrensen, GF, Koretz, J, Maraini, G, Harding, JJ 2000Opthalmologica21486CrossRefGoogle Scholar
  10. 10.
    Chylack Jr., LT 1984Ophthalmology91596Google Scholar
  11. 11.
    Bron, AJ, Sparrow, J, Brown, NA, Harding, JJ, Blakytny, R 1993Eye7260Google Scholar
  12. 12.
    Bettelheim, FA, Castoro, JA, White, O, Chylack Jr., LT 1986Curr. Eye Res.5925Google Scholar
  13. 13.
    Bettelheim, FA, Popdimitrova, N 1990Exp. Eye Res.50715CrossRefGoogle Scholar
  14. 14.
    Wang, H, Bettelheim, FA 1992Exp. Eye Res.55387CrossRefGoogle Scholar
  15. 15.
    Bettelheim, FA, Li, L, Zeng, FF 1998Res. Comm. Mol. Pathol. Pharmacol.1023Google Scholar
  16. 16.
    Kiss, M, Belagyi, J, Lőrinczy, D 2003J. Therm. Anal. Cal.72565CrossRefGoogle Scholar
  17. 17.
    Lőrinczy, D, Kiss, M, Belagyi, J 2003J. Therm. Anal. Cal.72573CrossRefGoogle Scholar
  18. 18.
    Sillinger, T, Than, P, Kocsis, B, Lőrinczy, D 2005J. Therm. Anal. Cal.82221CrossRefGoogle Scholar
  19. 19.
    Schäffer, B, Szakály, S, Lőrinczy, D 2005J. Therm. Anal. Cal.82537CrossRefGoogle Scholar
  20. 20.
    Biró, Zs, Nemes, V, Wittmann, I, Molnár, GA, Kocsis, B, Ludány, A 2003Szemészet14067in HungarianGoogle Scholar
  21. 21.
    Laemmli, UK 1970Nature27680CrossRefGoogle Scholar
  22. 22.
    Ishimitsu, S, Fujimoto, S, Ohara, A 1986J. Chromatogr.378222Google Scholar
  23. 23.
    Rácz, P, Hargitai, C, Alföldy, B, Bánki, P, Tompa, K 2000Exp. Eye Res.70529CrossRefGoogle Scholar
  24. 24.
    Bours, J, Fodisch, HJ, Hockwin, O 1987Ophthalmic Res.19235CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Biró Zs. 
    • 1
  • Nemes Vanda 
    • 1
  • Molnár G. A. 
    • 2
  • Wittmann I. 
    • 2
  • Ludány Andrea 
    • 3
  • Lőrinczy D. 
    • 4
  1. 1. Department of OphthalmologyUniversity of PécsPécsHungary
  2. 2.2nd Department of Medicine and Nephrological CenterUniversity of PécsPécsHungary
  3. 3.Department of Laboratory MedicineUniversity of PécsPécsHungary
  4. 4.Department of Biophysics, Faculty of MedicineUniversity of PécsPécsHungary

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