Journal of Sol-Gel Science and Technology

, Volume 71, Issue 2, pp 380–384 | Cite as

Synthesis of silica nanospheres with Ni2+-iminodiacetic acid for protein separation

  • Binjie Li
  • Yongna Zhang
  • Liuqin Cao
  • Yonghui Wu
  • Yanbao Zhao
Brief Communication


Silica (SiO2) nanospheres (NSs) with immobilized metal ligands have been prepared for the affinity separation of proteins. First, SiO2 NSs were prepared by controlled hydrolysis of tetraethoxysilane in a basic aqueous-ethanol solution. Then through reaction of iminodiacetic acid (IDA) with 3-glycidoxypropyltrimethoxysilane and immobilization of them onto the surfaces of above SiO2 NSs, novel affinity adsorbents with IDA chelating groups were obtained. After chelating Ni2+ ions, the SiO2–IDA–Ni2+ NSs were applied to separate his-tagged proteins directly from the mixture of lysed cells. The SiO2–IDA–Ni2+ NSs present negligible nonspecific protein adsorption and high protein binding ability (28.3 mg/g).


Silica nanospheres Surface modification Protein separation His-tagged protein 



Financial support of this work from National Natural Science Foundation of China (21271062) and Construction Fund of Henan province and Department of Education (SBGJ090515) is gratefully acknowledged.


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Binjie Li
    • 1
    • 2
  • Yongna Zhang
    • 3
  • Liuqin Cao
    • 3
  • Yonghui Wu
    • 2
  • Yanbao Zhao
    • 2
  1. 1.Institute of ImmunologyHenan UniversityKaifengPeople’s Republic of China
  2. 2.Key Laboratory for Special Functional MaterialsHenan UniversityKaifengPeople’s Republic of China
  3. 3.Department of Chemistry and Chemical EngineeringHenan UniversityKaifengPeople’s Republic of China

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