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Synthesis of silica nanospheres with Ni2+-iminodiacetic acid for protein separation

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Abstract

Silica (SiO2) nanospheres (NSs) with immobilized metal ligands have been prepared for the affinity separation of proteins. First, SiO2 NSs were prepared by controlled hydrolysis of tetraethoxysilane in a basic aqueous-ethanol solution. Then through reaction of iminodiacetic acid (IDA) with 3-glycidoxypropyltrimethoxysilane and immobilization of them onto the surfaces of above SiO2 NSs, novel affinity adsorbents with IDA chelating groups were obtained. After chelating Ni2+ ions, the SiO2–IDA–Ni2+ NSs were applied to separate his-tagged proteins directly from the mixture of lysed cells. The SiO2–IDA–Ni2+ NSs present negligible nonspecific protein adsorption and high protein binding ability (28.3 mg/g).

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Acknowledgments

Financial support of this work from National Natural Science Foundation of China (21271062) and Construction Fund of Henan province and Department of Education (SBGJ090515) is gratefully acknowledged.

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Authors and Affiliations

  1. Institute of Immunology, Henan University, Kaifeng, 475004, People’s Republic of China

    Binjie Li

  2. Key Laboratory for Special Functional Materials, Henan University, Kaifeng, 475004, People’s Republic of China

    Binjie Li, Yonghui Wu & Yanbao Zhao

  3. Department of Chemistry and Chemical Engineering, Henan University, Kaifeng, 475004, People’s Republic of China

    Yongna Zhang & Liuqin Cao

Authors
  1. Binjie Li
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  2. Yongna Zhang
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  3. Liuqin Cao
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  4. Yonghui Wu
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  5. Yanbao Zhao
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Correspondence to Yanbao Zhao.

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Li, B., Zhang, Y., Cao, L. et al. Synthesis of silica nanospheres with Ni2+-iminodiacetic acid for protein separation. J Sol-Gel Sci Technol 71, 380–384 (2014). https://doi.org/10.1007/s10971-014-3372-y

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  • DOI: https://doi.org/10.1007/s10971-014-3372-y

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