Clinically used radioprotectors, amifostine and cysteamine, have been shown to possess free-radical scavenging activities. Natural antioxidants also possess free-radical scavenging activities, some of which have been shown to have radioprotective capability. We carried out a quantitative determination of the scavenging abilities in amifostine and cysteamine and compared the potencies with natural antioxidants by using multiple free-radical scavenging method. Overall, amifostine and cysteamine showed scavenging activity as potent as natural antioxidants. We found a correlation between scavenging activity and radioprotective efficiency that was previously determined in rat thymocytes. These correlations are indicative that natural antioxidants can be effective radioprotectors.
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Anuranjani BM (2014) Concerted action Nrf2-ARE pathway, MRN complex, HMGB1 and inflammatory cytokines—implication in modification of radiation damage. Redox Biol 2:832–846
Zhou R, Zhang H, Wang Z, Li J, Zhou X, Gan L, Liu Y (2014) The effects of X-ray radiation on the eye development of zebrafish. Hum Exp Toxicol 33:1040–1050
Azzam EI, Jay-Gerin JP, Pain D (2012) Ionizing radiation-induced metabolic oxidative stress and pro-loged cell injury. Cancer Lett 327:48–60
Szumiel I (2015) Ionizing radiation-induced oxidative stress, epigenetic changes and genomic instability: the pivotal role of mitochondria. Int J Radiat Biol 91:1–12
Nakano T, Xu X, Salem AM, Shoulkamy MI, Ide H (2017) Radiation-induced DNA-protein cross-links: mechanisms and biological significance. Free Radiat Biol Med 107:136–145
Kouvaris JR, Kouloulias VE, Vlahos LJ (2007) Amifostine: the first selective-target and broad-spectrum radioprotector. Oncologist 12:738–747
Sekine-Suzuki E, Nakanishi I, Shimokawa T, Ueno M, Matsumoto K, Murakami T (2013) High-throughput screening of radioprotectors using rat thymocytes. Anal Chem 85:7650–7653
Yahyapour R, Shabeeb D, Cheki M, Musa AE, Farhood B, Rezaeyan A, Amini P, Fallah H, Najafi M (2018) Radiation protection and mitigation by natural antioxidants and flavonoids: implications to radiotherapy and radiation disasters. Curr Mol Pharm 11:285–304
Ertekin MV, Sezen O (2007) Radioprotective effects of antioxidants. Panglossi HV (ed) Nova Sci Publishers, Inc, New York
Oowada S, Endo N, Kameya H, Shimmei M, Kotake Y (2012) Multiple free-radical scavenging capacity in serum. J Clin Biochem Nutr 51:117–121
Sueishi Y, Hori M, Ishikawa M, Matsu-ura K, Kamogawa E, Honda Y, Kita M, Ohara K (2014) Scavenging rate constants of hydrophilic antioxidants against multiple reactive oxygen species. J Clin Biochem Nutr 54:67–74
Sueishi Y, Nii R, Kakizaki N (2017) Resveratrol analogues like piceatannol are potent antioxidants as quantitatively demonstrated through the high scavenging ability against reactive oxygen species and methyl radical. Bioorg Med Chem Lett 27:5203–5206
Sueishi Y, Nii R (2018) Monoterpene’s multiple free radical scavenging capacity as compared with the radioprotective agent cysteamine and amifostine. Bioorg Med Chem Lett 28:3031–3033
Sueishi Y, Masamoto H, Kotake Y (2019) Heat treatments of ginger root modify but not diminish its antioxidant activity as measured with multiple free radical scavenging (MULTIS) method. J Clin Biochem Nutr 64:143–147
Kohri S, Fujii H, Oowada S, Endo N, Sueishi Y, Kusakabe M, Shimmei M, Kotake Y (2009) An oxygen radical absorbance capacity-like assay that directly quantifies the antioxidant’s scavenging capacity against AAPH-derived free radicals. Anal Biochem 386:167–171
Prior RL, Hoang H, Gu L, Wu X, Bacchiocca M, Howard L, Hampsh-Woodill M, Huang D, Ou B, Jacob R (2003) Assays for hydrophilic and lipophilic antioxidant capacity (oxygen radical absorbance capacity (ORACFL)) of plasma and other biological and food samples. J Agric Food Chem 51:3273–3279
Aprotosoaie AC, Trifan A, Gille E, Petreus T, Bordeianu G, Miron A (2015) Can phytochemicals be a bridge to develop new radioprotective agents? Phytochem Rev 14:555–566
Sekine-Suzuki E, Nakanishi I, Imai K, Ueno M, Shimokawa T, Matsumoto K, Fukuhara K (2018) Efficient protective activity of a planar catechin analogue against radiation-induced apoptosis in rat thymocytes. RSC Adv 8:10158–10162
Aizawa Y, Sunada S, Hirakawa H, Fujimori A, Kato TA, Uesaka M (2018) Design and evaluation of a novel flavonoid-based radioprotective agent utilizing monoglucosyl rutin. J Radiat Res 59:272–281
Hall EJ, Giaccia AJ (eds) (2012) Radiobiology for the radiologist, 7th edn. Lippincott Williams & Wilkins, Philadelphia, pp 3–11
We thank Dr. Yashige Kotake for helpful discussion and critical reading of the manuscript.
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Sueishi, Y., Fujii, T. & Nii, R. Free-radical scavenging activity of radioprotectors: comparison between clinically used radioprotectors and natural antioxidants. J Radioanal Nucl Chem 325, 695–700 (2020). https://doi.org/10.1007/s10967-020-07258-7
- Natural antioxidant
- Antioxidant ability
- Radioprotective effect
- ESR spin trapping