99mTc-Labeled human and camel lactoferrin for detection of Staphylococcus aureus infections
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Human and camel lactoferrin, were labeled with technetium-99m using a simple technique that yielded a labelling efficiency of 98% and stable complexes over time. The camel Lactoferrin was purified. The proteins were investigated for targeting infections in experimental animals. The biodistribution of those labeled proteins in mice infected with Staphylococcus aureus at the femoral muscle was investigated and test ratios of 3.43 and 3.71 were obtained after 60 min for the camel and the human Lactoferrin, respectively. These values were compared to each other and to those reported in the literature, leading us to conclude that the radiotracers are efficient.
Keywords99mTechnetium Staphylococcus aureus Human lactoferrin Camel lactoferrin Infectious sites
The authors would like to thank the staff of Pasteur Institute of Tunisia for their cooperation and assistance with some. The authors are also grateful to Pr BEN SLIMANE of the Salah Azaiez Institute of Tunis for generously providing us with radioactive technetium.
- 5.Nibbering PH, Ravensbergen E, Welling MM, van Berkel LA, van Berkel PHC, Pauwels EKJ, Nuijens JH (2001) Human lactoferrin and peptides derived from its N terminus are highly effective against infections with antibiotic-resistant bacteria. Infect Immun 69(3):1469–1476CrossRefPubMedPubMedCentralGoogle Scholar
- 14.Rusckowski M, Gupta S, Liu G, Dou S, Hnatowich DJ (2004) Investigation of a 99mTc-labelled bacteriophages as a potential infection-specific imaging agent. J Nucl Med 45:13–14Google Scholar
- 17.Belhadj-Tahar H, Coulais Y, Cros G, Darbieu MH, Tafani JA, Fabre J, Esquerré JP, Guiraud R (1996) Technetium labeling of Bi, Tri and tetradentate ligands derived from 2 aminocyclopentene-1-dithiocarboxylic acid: characterization and biodistribution of their oxo and nitrido 99mTechnetium complexes. J Nucl Med Biol 23:353–357CrossRefGoogle Scholar
- 21.Nibbering PH, Ravensbergen E, Welling MM, van Berkel A, van Berkel PHC, Pauwels EKJ, Nuijens JH (2001) Human lactoferrin and peptides derived from its N terminus are highly effective against infections with antibiotic-resistant bacteria. Infect Immun 69:1469–1476CrossRefPubMedPubMedCentralGoogle Scholar
- 23.Singh AK, Verma J, Bhatnagar A, Sen S, Bose M (2003) Tc-99m isoniazid: a specific agent for diagnosis of tuberculosis. World J Nucl Med 2:292–305Google Scholar