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99mTc-Labeled human and camel lactoferrin for detection of Staphylococcus aureus infections

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Abstract

Human and camel lactoferrin, were labeled with technetium-99m using a simple technique that yielded a labelling efficiency of 98% and stable complexes over time. The camel Lactoferrin was purified. The proteins were investigated for targeting infections in experimental animals. The biodistribution of those labeled proteins in mice infected with Staphylococcus aureus at the femoral muscle was investigated and test ratios of 3.43 and 3.71 were obtained after 60 min for the camel and the human Lactoferrin, respectively. These values were compared to each other and to those reported in the literature, leading us to conclude that the radiotracers are efficient.

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Notes

  1. Albino Wistar male mice weighing 22-34 g obtained from Pasteur institute of Tunis and housed for 1 day before experiments in our laboratory. Mice were treated according to the Ethical Principles and Guidelines for Experiments on Animals (3rd edition 2005). [Swiss Academy of Medical Sciences (SAMS) Swiss Academy of Sciences (SCNAT)].

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Acknowledgements

The authors would like to thank the staff of Pasteur Institute of Tunisia for their cooperation and assistance with some. The authors are also grateful to Pr BEN SLIMANE of the Salah Azaiez Institute of Tunis for generously providing us with radioactive technetium.

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Authors and Affiliations

  1. Research Unit, Application of Nuclear Techniques in the Fields of Health, Agriculture and Environment, National Centre for Nuclear Science and Technology (CNSTN), Sidi Thabet Technopark, 2020, Ariana, Tunisia

    Alifa Kanoun, Issam B. Salem, Amine Asmi & Mouldi Saidi

  2. Corporation of Pharmaceutical Industry Ibn Al Baytar, 11 Rue 8610 Zone Industrielle, 2035, La Soukra, Tunisia

    Alifa Kanoun & Frida Darghouth

  3. Faculty of Sciences of Bizerte, 7021, Zarzouna, Tunisia

    Alifa Kanoun

  4. Laboratoire Elevage et faune Sauvage, Institut des Régions Arides, 4119, Medenine, Tunisia

    Halima El Hatmi

  5. ISARA Lyon, BioDyMIA (Bioingénierie et Dynamique Microbienne aux Interfaces Alimentaires), Univ Lyon, Université Claude Bernard Lyon 1, Equipe Mixte d’Accueil n°3733, IUT Lyon 1, 01000, Bourg en Bresse, France

    Isabelle ADT

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  1. Alifa Kanoun
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  2. Issam B. Salem
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  7. Mouldi Saidi
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Correspondence to Alifa Kanoun.

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Kanoun, A., Salem, I.B., Darghouth, F. et al. 99mTc-Labeled human and camel lactoferrin for detection of Staphylococcus aureus infections. J Radioanal Nucl Chem 317, 177–185 (2018). https://doi.org/10.1007/s10967-018-5924-z

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  • DOI: https://doi.org/10.1007/s10967-018-5924-z

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