Xylenol orange probe-based spectroscopic insight into the interaction between strontium (II) and bovine serum albumin
- 74 Downloads
The mechanism of the interaction between Sr(II) and bovine serum albumin (BSA) in aqueous solution was investigated using xylenol orange (XO) as a probe by UV–Vis absorption and fluorescence spectroscopy. Binding ratios of n Sr(II):n XO = 1:1 and n Sr(II):n XO:n BSA = 1:1:1 (pH 7.4) were confirmed. Static quenching occurred between Sr(II)-XO and BSA because of hydrophobic force. Based on the Förster theory of nonradioactive energy transfer, the reactive distance and energy transfer efficiency between Sr(II)-XO and BSA at room temperature were determined. The thermodynamic data obtained indicate that the formation of Sr(II)-XO–BSA complex is spontaneous and driven by entropy and enthalpy.
KeywordsSr(II) Bovine serum albumin Interaction mechanism UV–Vis spectroscopy Fluorescence spectroscopy Xylenol orange
Funding for this work was supported by the PhD Foundation of Southwest University of Science and Technology (granted NO. 13zx7132).
- 10.Tao Y, Xu FW, Cao SH, Li JZ, Xu FD (2000) Development of hygienic standard for Strontium in drinking water. China Public Health 16:615Google Scholar
- 31.Han MX, Zou XP, Li YQ (2009) Research on the Interaction between Hg(II) and Bovine Serum Albumin by Fluorescence Spectroscopy. Chin J Spectrosc Lab 26:173–176Google Scholar
- 35.Huang JH, Ma HM, Sun ST, Chen X, Dong HX, Wei Q (2006) Study on the mechanism of interaction between TH-PF-Mo(VI) complex and bovine serum albumin by fluorimetric method. Spectrosc Spect Anal 26:1899–1902Google Scholar
- 36.Yang P, Yang MM, Yang BS (1996) Fluorescence enhancement effect and the interaction between donor and acceptor. Chin J Chem 14:9–113Google Scholar
- 37.Bian HD, Li M, Yu Q, Liang H (2006) Studies on the interaction between NaVO3 and bovine serum albumin by fluorescence method. Chin J Inorg Chem. 22:845–850Google Scholar