Compartmentalization of Co and Mn in live cells of Escherichia coli: investigation using 60Co and 54Mn as radioindicators
Compartmentalization of Co and Mn in Escherichia coli BL21 (D3) was investigated using 60Co and 54Mn as radioindicators. Living non-growing E. coli cells sequestered accumulated cobalt and manganese not only on cell surface but significant amounts of uptaken metals were associated with periplasmic space and cytoplasm. Both Co and Mn cell compartmentalization was time and concentration dependent process although total binding capacity of cells for Co was significantly lower compared to Mn. This should be considered in sorption studies especially in cases when metal recovery from bacterial cells is required or bacteria should be used in several sorption–desorption cycles.
Keywords60Co 54Mn Escherichia coli Uptake Cell compartmentalization
This work was supported by the Slovak Research and Development Agency under the contract No. APVV-0380-12.
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