In-house preparation of macroaggregated albumin (MAA) for 68Ga labeling and its comparison with commercially available MAA
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99mTc labeled macroaggregated albumin (MAA) is used worldwide for SPECT imaging of lungs, using commercially available MAA kits. An attempt was made to indigenously prepare MAA for radiolabeling with 68Ga, for PET imaging. MAA particles (59.9 ± 18.1 µm) were successfully prepared in-house and radiolabeled with 68Ga. Retention of in-house synthesized 68Ga-MAA in lungs of Swiss mice (71.6 ± 2.1 %ID) was similar to that of 68Ga-MAA prepared using commercial kits (83.5 ± 2.3 %ID). In vivo Cherenkov luminescent imaging of in-house prepared 68Ga-MAA in Swiss mice at 15 min and 60 min p.i. showed maximum retention of activity in lungs and negligible leaching into liver.
Keywords68Ga Macroaggregated albumin Positron emission tomography (PET) Cherenkov imaging
Research at the Bhabha Atomic Research Centre (BARC) is part of the ongoing activities of the Department of Atomic Energy, India and is fully supported by government funding. The authors express their sincere thanks to Dr. K.L. Ramakumar, Director, Radiochemistry & Isotope Group, BARC for his support and encouragement. Thanks are also due to Dr. Rubel Chakravarty, Isotope Production & Applications Division, BARC for providing 68GaCl3 from the in-house generator and to Dr. D.K. Maurya, Radiation Biology & Health Sciences Division, BARC for his help in carrying out particle size analysis.
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