Solid phase extraction of uranium(VI) on phosphorus-containing polymer grafted 4-aminoantipyrine

  • Mustafa Soylak
  • Mansoor Khan
  • Rasim Alosmanov
  • Jasmin Shah
  • Mohammad Rasul Jan


Phosphorus-containing polymer grafted 4-aminoantipyrine has been synthesized and used for solid phase extraction of U(VI) prior to its UV–Visible spectrophotometric determination by using arsenazo(III). The adsorbent was characterized by using FT-IR and SEM analysis. The influence of parameters including pH, adsorbent dose, amount of complexing reagent, sample volume and matrix effect have been optimized. The detection limit was determined as 1.4 µg L−1 with preconcentration factor of 30 and RSD of 1.4 %. The accuracy was checked by the analysis of GBW07424 soil and TMDA-64.2 environmental water certified reference materials. The method was applied to natural water and soil samples.


Solid phase extraction Spectrophotometry Determination Uranium Arsenazo(III) 



Mansoor Khan is thankful to the Scientific and Technological Research Council of Turkey (TUBITAK) under “2216 Research Fellowship Programme for Foreign Citizens” for financial support. Authors are also thanks to Erkan Yilmaz for his helps.


  1. 1.
    Donia AM, Atia AA, Moussa EMM, El-Sherif AM, Abd El-Magied MO (2009) Removal of uranium(VI) from aqueous solutions using glycidyl methacrylate chelating resins. Hydrometall 95:183–189CrossRefGoogle Scholar
  2. 2.
    Shuibo X, Chun Z, Xinghuo Z, Jing Y, Xiaojian Z, Jingsong W (2009) Removal of uranium (VI) from aqueous solution by adsorption of hematite. J Environ Radioact 100:162–166CrossRefGoogle Scholar
  3. 3.
    WHO (2011) Guidelines for drinking-water quality, 4th edn. World Health Organization, GenevaGoogle Scholar
  4. 4.
    Garbos S, Swiecicka D (2015) Application of bimodal distribution to the detection of changes in uranium concentration in drinking water collected by random daytime sampling method from a large water supply zone. Chemosphere 138:377–382CrossRefGoogle Scholar
  5. 5.
    Danko B, Dybczyński R (1997) Determination of molybdenum and uranium in biological materials by radiochemical neutron activation analysis. J Radioanal Nucl Chem 216:51–57CrossRefGoogle Scholar
  6. 6.
    Kim KH, Burnett WC (1983) Gamma-ray spectrometric determination of uranium-series nuclides in marine phosphorites. Anal Chem 55:1796–1800CrossRefGoogle Scholar
  7. 7.
    Kuznetsov S, Hayashi H, Minato K, Gaune-Escard M (2006) Electrochemical transient techniques for determination of uranium and rare-earth metal separation coefficients in molten salts. Electrochim Acta 51:2463–2470CrossRefGoogle Scholar
  8. 8.
    Campen W, Bächmann K (1979) Laser-induced fluorescence for the direct determination of small concentrations of uranium in water. Microchim Acta 72:159–170CrossRefGoogle Scholar
  9. 9.
    Oshita K, Oshima M, Gao Y, Lee K-H, Motomizu S (2003) Synthesis of novel chitosan resin derivatized with serine moiety for the column collection/concentration of uranium and the determination of uranium by ICP-MS. Anal Chim Acta 480:239–249CrossRefGoogle Scholar
  10. 10.
    Shah F, Soylak M, Kazi TG, Afridi HI (2013) Development of an extractive spectrophotometric method for uranium using MWCNTs as solid phase and arsenazo(III) as chromophore. J Radioanal Nucl Chem 296:1239–1245CrossRefGoogle Scholar
  11. 11.
    Kaykhaii M, Ghasemi E (2013) Room temperature ionic liquid-based dispersive liquid–liquid microextraction of uranium in water samples before spectrophotometric determination. Anal Methods 5:5260–5266CrossRefGoogle Scholar
  12. 12.
    Aydin FA, Soylak M (2007) Solid phase extraction and preconcentration of uranium(VI) and thorium(IV) on Duolite XAD761 prior to their inductively coupled plasma mass spectrometric determination. Talanta 72:187–192CrossRefGoogle Scholar
  13. 13.
    Madrakian T, Afkhami A, Mousavi A (2007) Spectrophotometric determination of trace amounts of uranium(VI) in water samples after mixed micelle-mediated extraction. Talanta 71:610–614CrossRefGoogle Scholar
  14. 14.
    Ru Y, Yan L, Guilan S, Tao W, Jiaomai P (1995) Spectrophotometric determination of uranium in natural water with the new chromogenic reagent p-carboxychlorophosphonazo. Anal Chim Acta 314:95–99CrossRefGoogle Scholar
  15. 15.
    Jalbani N, Soylak M (2014) Spectrophotometric determination of uranium using chromotrope 2R complexes. J Radioanal Nucl Chem 301:263–268CrossRefGoogle Scholar
  16. 16.
    Florence T, Anal Y (1963) Spectrophotometric determination of uranium with 4-(2-Pyridylazo) resorcinol. Chem 35:1613–1616Google Scholar
  17. 17.
    Rajan M, Shinde V (1996) Synergistic extraction and separation studies of uranium(VI). J Radioanal Nucl Chem 203:169–176CrossRefGoogle Scholar
  18. 18.
    Shariati S, Yamini Y, Zanjani MK (2008) Simultaneous preconcentration and determination of U(VI), Th(IV), Zr (IV) and Hf(IV) ions in aqueous samples using micelle-mediated extraction coupled to inductively coupled plasma-optical emission spectrometry. J Hazard Mater 156:583–590CrossRefGoogle Scholar
  19. 19.
    Singh BN, Maiti B (2006) Separation and preconcentration of U(VI) on XAD-4 modified with 8-hydroxy quinoline. Talanta 69(2006):393–396CrossRefGoogle Scholar
  20. 20.
    Sadeghi S, Sheikhzadeh E (2009) Solid phase extraction using silica gel modified with murexide for preconcentration of uranium(VI) ions from water samples. J Hazard Mater 163:861–868CrossRefGoogle Scholar
  21. 21.
    Starvin AM, Rao TP (2004) Solid phase extractive preconcentration of uranium(VI) onto diarylazobisphenol modified activated carbon. Talanta 63:225–232CrossRefGoogle Scholar
  22. 22.
    Zhai Y, Liu Y, Chang X, Chen S, Huang X (2007) Selective solid-phase extraction of trace cadmium(II) with an ionic imprinted polymer prepared from a dual-ligand monomer. Anal Chim Acta 593:123–128CrossRefGoogle Scholar
  23. 23.
    Metilda P, Sanghamitra KJ, Gladis JM, Naidu G, Rao TP (2005) Amberlite XAD-4 functionalized with succinic acid for the solid phase extractive preconcentration and separation of uranium (VI). Talanta 65:192–200Google Scholar
  24. 24.
    Duran A, Tuzen M, Soylak M (2009) Preconcentration of some trace elements via using multiwalled carbon nanotubes as solid phase extraction adsorbent. J Hazard Mater 169:466–471CrossRefGoogle Scholar
  25. 25.
    Praveen R, Metilda P, Daniel S, Rao TP (2005) Solid phase extractive preconcentration of uranium(VI) using quinoline-8-ol anchored chloromethylated polymeric resin beads. Talanta 67:960–967CrossRefGoogle Scholar
  26. 26.
    Kütahyalı C, Eral M (2004) Selective adsorption of uranium from aqueous solutions using activated carbon prepared from charcoal by chemical activation. Sep Purif Technol 40:109–114CrossRefGoogle Scholar
  27. 27.
    Yousefi SR, Ahmadi SJ, Shemirani F, Jamali MR, Salavati-Niasari M (2009) Simultaneous extraction and preconcentration of uranium and thorium in aqueous samples by new modified mesoporous silica prior to inductively coupled plasma optical emission spectrometry determination. Talanta 80:212–217CrossRefGoogle Scholar
  28. 28.
    Aytas SO, Akyil S, Eral M (2004) Adsorption and thermodynamic behavior of uranium on natural zeolite. J Radioanal Nucl Chem 260:119–125CrossRefGoogle Scholar
  29. 29.
    Wang G, Liu J, Wang X, Xie Z, Deng N (2009) Adsorption of uranium(VI) from aqueous solution onto cross-linked chitosan. J Hazard Mater 168:1053–1058CrossRefGoogle Scholar
  30. 30.
    Zhu X, Cui Y, Chang X, Zou X, Li Z (2009) Selective solid-phase extraction of lead(II) from biological and natural water samples using surface-grafted lead(II)-imprinted polymers. Microchim Acta 164:125–132CrossRefGoogle Scholar
  31. 31.
    Buyuktiryaki S, Say R, Ersöz A, Birlik E, Denizli A (2005) Selective preconcentration of thorium in the presence of UO2 2+, Ce3+ and La3+ using Th(IV)-imprinted polymer. Talanta 67:640–645CrossRefGoogle Scholar
  32. 32.
    Prasad BB, Sharma PS, Lakshmi D (2007) Molecularly imprinted polymer-based solid-phase extraction combined with molecularly imprinted polymer-based sensor for detection of uric acid. J Chromatogr 1173A:18–26CrossRefGoogle Scholar
  33. 33.
    Baggiani C, Anfossi L, Giovannoli C (2007) Solid phase extraction of food contaminants using molecular imprinted polymers. Anal Chim Acta 591:29–39CrossRefGoogle Scholar
  34. 34.
    Yilmaz E, Alosmanov R, Soylak M (2015) Magnetic solid phase extraction of lead(II) and cadmium(II) on a magnetic phosphorus-containing polymer (M-PhCP) for their microsampling flame atomic absorption spectrometric determinations. RSC Adv 5:33801–33808CrossRefGoogle Scholar
  35. 35.
    Wang Y, Han J, Liu Y, Wang L, Ni L, Tang X (2016) Recyclable non-ligand dual cloud point extraction method for determination of lead in food samples. Food Chem 190:1130–1136CrossRefGoogle Scholar
  36. 36.
    Dogan S, Kaya FND, Atakol O (2015) Enrichment of copper and nickel with solid phase extraction using multiwalled carbon nanotubes modified with Schiff bases. Int J Environ Anal Chem 95:698–712CrossRefGoogle Scholar
  37. 37.
    Soleimani M, Rafiei B, Siahpoosh ZH (2015) Ghezeljeh montmorillonite nanoclay as a natural adsorbent for solid phase extraction of copper ions from food samples. J Anal Chem 70:794–803CrossRefGoogle Scholar
  38. 38.
    Alothman ZA, Yilmaz E, Habila M, Soylak M (2015) Solid phase extraction of metal ions in environmental samples on 1-(2-pyridylazo)-2-naphthol impregnated activated carbon cloth. Ecotoxicol Environ Saf 112:74–79CrossRefGoogle Scholar
  39. 39.
    Mellah A, Chegrouche S, Barkat M (2006) The removal of uranium(VI) from aqueous solutions onto activated carbon: kinetic and thermodynamic investigations. J Colloid Interface Sci 296:434–441CrossRefGoogle Scholar
  40. 40.
    Soylak M, Ercan O (2009) Selective separation and preconcentration of copper(II) in environmental samples by the solid phase extraction on multi-walled carbon nanotubes. J Hazard Mater 168:1527–1531CrossRefGoogle Scholar
  41. 41.
    Ghaedi M, Ahmadi F, Tavakoli Z, Montazerozohori M, Khanmohammadi A, Soylak M (2008) Three modified activated carbons by different ligands for the solid phase extraction of copper and lead. J Hazard Mater 152:1248–1255CrossRefGoogle Scholar
  42. 42.
    Barfi B, Rajabi M, Asghari A (2016) A simple organic solvent-free liquid-liquid microextraction method for the determination of potentially toxic metals as 2-(5-bromo-2-pyridylazo)-5-(diethylamino)phenol complex from food and biological samples. Biol Trace Elem Res. doi: 10.1007/s12011-015-0489-y Google Scholar
  43. 43.
    Soylak M, Elci L, Dogan M (1996) Determination of some trace metal impurities in refined and unrefined salts after preconcentration onto activated carbon. Fresenius Environ Bull 5:148–155Google Scholar
  44. 44.
    Zadeh HRM, Ahmadvand P, Behbahani A, Amini MM, Sayar O (2016) Dithizone-modified graphene oxide nano-sheet as a sorbent for pre-concentration and determination of cadmium and lead ions in food. Food Addit Contam A. doi: 10.1080/19440049.2015.1066937 Google Scholar
  45. 45.
    Bahar S, Karami F (2016) Amino-functionalized Fe3O4-graphene oxide nanocomposite as magnetic solid-phase extraction adsorbent combined with flame atomic absorption spectrometry for copper analysis in food samples. Iran Chem Soc. doi: 10.1007/s13738-015-0699-4 Google Scholar
  46. 46.
    Aydin FA, Soylak M (2010) Separation, preconcentration and inductively coupled plasma-mass spectrometric (ICP-MS) determination of thorium(IV), titanium(IV), iron(III), lead(II) and chromium(III) on 2-nitroso-1-naphthol impregnated MCI GEL CHP20P resin. J Hazard Mater 173:669–674CrossRefGoogle Scholar
  47. 47.
    Kandhro GA, Kazi TG, Soylak M (2014) Solid phase extraction of thorium on multiwalled carbon nanotubes prior to UV-Vis spectrophotmetric determination in ore samples. At Spectrosc 35:270–274Google Scholar
  48. 48.
    Gouda AA (2016) A new coprecipitation method without carrier element for separation and preconcentration of some metal ions at trace levels in water and food samples. Talanta 146:435–441CrossRefGoogle Scholar
  49. 49.
    Soylak M, Divrikli U, Elci L, Dogan M (1998) Column chromatographic preconcentration of copper, nickel and iron in ammonium salts by graphite furnace atomic absorption spectrometry. Kuwait J Sci Eng 25:389–396Google Scholar
  50. 50.
    Altunay N, Gürkan R, Orhan U (2015) A new ultrasonic-assisted cloud-point extraction procedure for pre-concentration and determination of ultra-trace levels of copper in selected beverages and foods by flame atomic absorption spectrometry. Food Addit Contam A 32:1475–1487CrossRefGoogle Scholar
  51. 51.
    El Himri M, Pastor A, de la Guardia M (2000) Determination of uranium in tap water by ICP-MS. Fresenius J Anal Chem 367:151–156CrossRefGoogle Scholar
  52. 52.
    Liu Y, Cao X, Le Z, Luo M, Xu W, Huang G (2010) Pre-concentration and determination of trace uranium(VI) in environments using ion-imprinted chitosan resin via solid phase extraction. J Braz Chem Soc 21:533–540CrossRefGoogle Scholar
  53. 53.
    Gladis JM, Prasada RT (2002) Solid phase extractive preconcentration of uranium on to 5, 7-dichloroquinoline-8-ol modified naphthalene. Anal Lett 35:501–515CrossRefGoogle Scholar
  54. 54.
    Preetha C, Prasada RT (2003) Preparation of 1-(2-pyridylazo)-2-naphthol functionalized benzophenone/naphthalene and their uses in solid phase extractive preconcentration/separation of uranium (VI). Radiochim Acta 91:247–252CrossRefGoogle Scholar
  55. 55.
    Rao TP, Metilda P, Gladis JM (2006) Preconcentration techniques for uranium(VI) and thorium(IV) prior to analytical determination—an overviewç. Talanta 68:1047–1064CrossRefGoogle Scholar
  56. 56.
    Zhao Y, Liu C, Feng M, Chen Z, Tian G, Wang L, Huang J, Li S (2010) Solid phase extraction of uranium(VI) onto benzoylthiourea-anchored activated carbon. J Hazard Mater 176:119–124CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest, Hungary 2015

Authors and Affiliations

  • Mustafa Soylak
    • 1
  • Mansoor Khan
    • 1
    • 2
  • Rasim Alosmanov
    • 3
  • Jasmin Shah
    • 2
  • Mohammad Rasul Jan
    • 2
  1. 1.Faculty of Sciences, Department of ChemistryErciyes UniversityKayseriTurkey
  2. 2.Institute of Chemical SciencesUniversity of PeshawarPeshawarPakistan
  3. 3.Chemistry DepartmentBaku State UniversityBakuAzerbaijan

Personalised recommendations