Journal of Radioanalytical and Nuclear Chemistry

, Volume 307, Issue 2, pp 1253–1263 | Cite as

Determination of uranium(VI) using Penicillium chrysogenum immobilized on silica gel and spectrophotometer

  • D. K. Kulal
  • A. V. Pansare
  • S. R. Tetgure
  • M. Karve
  • V. R. Patil


A solid phase extraction method for separation and enrichment of uranium(VI) was developed using Penicillium chrysogenum immobilized on silica mixed with double distilled water. Conditions such as pH, sample flow rate and volume, eluting solution and interfering ions with uranium(VI) optimized and determined spectrophotometrically for quantitative sorbtion. Maximum adsorption found at monolayer was 1.3004 mg/g using Langmuir isotherm. The limits of detection (LOD) and limit of quantification (LOQ) of the proposed method for uranium(VI) was 0.61 μg/L and was 2.02 μg/L respectively. The method developed for the separation and recovery of uranium(VI) was applied sediment samples and tap water samples.


Solid phase extraction Penicillium chrysogenum Uranium(VI) Langmuir adsorption isotherm 



The Authors would like to acknowledge the Department of Chemistry, University of Mumbai, and Indian Institute of technology (IIT), Mumbai, India for providing the research facilities.


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Copyright information

© Akadémiai Kiadó, Budapest, Hungary 2015

Authors and Affiliations

  • D. K. Kulal
    • 1
  • A. V. Pansare
    • 1
  • S. R. Tetgure
    • 1
  • M. Karve
    • 1
  • V. R. Patil
    • 1
  1. 1.Department of ChemistryUniversity of MumbaiMumbaiIndia

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