Preparation, quality control and biological characterization of 99mTc-vincristine

  • Saira Hina
  • Samina Roohi
  • Muhammad Ibrahim Rajoka
  • Asma Haque
  • Tanveer Hussain Bokhari
  • Muhammad Sohaib


In present study it is aimed to radiolabel vincristine with 99mTc and to evaluate bioaffinity of 99mTc labeled vinc. The optimum conditions required to obtain 99.6 ± 0.4 %, (n = 5) radiolabeling yield of 99mTc-vincristine (99mTc-vinc) were as follows: pH 4, 5 µg of vincristine sulphate, 6 µg SnCl2·2H2O as a reducing agent and 10 min incubation time at room temperature. Quality control of 99mTc-vinc was done by using paper electrophoresis and thin layer chromatography. The radiolabeling yield was confirmed by High performance liquid chromatography using radioactive and UV detector operating at 230 nm. 99mTc-vinc was stable in vitro for 5 h. Biodistribution and scintigraphy of 99mTc-vinc was performed in mice and rabbits respectively and that 99mTc-vinc showed high uptake of it in liver and spleen. Finally 99mTc-vinc may be the potential imaging agent for liver and spleen.


99mTc-vincristine Quality control SPECT imaging Biodistribution Scintigraphy 



The authors are thankful to Mr. Ibrar Haider, Zafar, Riaz and Jamshed for their helpful discussions and providing facilities to work properly in lab.


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Copyright information

© Akadémiai Kiadó, Budapest, Hungary 2014

Authors and Affiliations

  • Saira Hina
    • 1
  • Samina Roohi
    • 2
  • Muhammad Ibrahim Rajoka
    • 1
  • Asma Haque
    • 1
  • Tanveer Hussain Bokhari
    • 3
  • Muhammad Sohaib
    • 4
  1. 1.Department of Bioinformatics and BiotechnologyGovernment College University FaisalabadFaisalabadPakistan
  2. 2.Isotope Production DivisionPakistan Institute of Nuclear Science and TechnologyIslamabadPakistan
  3. 3.Department of ChemistryGovernment College University FaisalabadFaisalabadPakistan
  4. 4.Pakistan Institute of Engineering and Applied SciencesIslamabadPakistan

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