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Bioevaluation of 99mTc(CO)3–Guanine in vitro and in vivo

  • Çiğdem İçhedef
  • Serap Teksöz
  • Perihan Ünak
  • Kamile Şenocak
  • Emin İ. Medine
  • Türkan Ertay
  • Recep Bekiş
Article
  • 79 Downloads

Abstract

The aim of this study is to examine biological behaviour of radiolabeled guanine with [Tc(CO)3]+ core in vitro and in vivo. In vitro biological behavior of 99mTc(CO)3–Gua was evaluated on Lung (A-549), Breast (MCF-7), Colonic (Caco) carcinoma cell lines and normal human bronchial epithelial (NHBE). 99mTc(CO)3–Gua compound showed high uptake on A-549 cell line when compared to NHBE cell line. Biodistribution characteristics of 99mTc(CO)3–Gua was evaluated using New Zeland Rabbits. Scintigraphic results showed that a high level of radioactivity was observed in the lungs and liver shortly after administration of the 99mTc(CO)3–Gua and excretion takes place via both renal and hepatobiliary route. It was concluded that 99mTc(CO)3–Gua could be used as a nucleotide radiopharmaceutical for imaging purposes.

Keywords

Guanine 99mTechnetium Cell culture Imaging 

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Copyright information

© Akadémiai Kiadó, Budapest, Hungary 2011

Authors and Affiliations

  • Çiğdem İçhedef
    • 1
  • Serap Teksöz
    • 1
  • Perihan Ünak
    • 1
  • Kamile Şenocak
    • 1
  • Emin İ. Medine
    • 1
  • Türkan Ertay
    • 2
  • Recep Bekiş
    • 2
  1. 1.Institute of Nuclear SciencesEge UniversityİzmirTurkey
  2. 2.Department of Nuclear Medicine, School of MedicineDokuz Eylul UniversityInciralti İzmirTurkey

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