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Radiocharacterization of the 99mTc–rufloxacin complex and biological evaluation in Staphylococcus aureus infected rat model

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Abstract

99mTc–rufloxacin (99mTc–RUN) complex was prepared by reaction of different amounts of reduced sodium pertechnetate with different amount of Rufloxacin (RUN) antibiotic for the in vivo scintigraphic localization of the Staphylococcus aureus (S. aureus) infectious foci in Male Wister Rats (MWR) model. The 99mTc–RUN complex was radiochemically and biologically characterized in terms of radiochemical stability in saline, serum, in vitro binding with S. aureus and biodistribution in artificially infected with S. aureus MWR. The 99mTc–RUN complex showed stability more than 90% up to 240 min in normal saline with a maximum stability value of 98.10 ± 0.18% at 30 min after reconstitution. At 37 °C the complex showed in vitro permanence in serum up to 16 h with 13.90% side products during incubation. The 99mTc–RUN complex showed saturated in vitro binding with S. aureus at different intervals with a maximum uptake value of 71.50%. Infected to normal muscle, infected to inflamed and inflamed to normal muscles ratios were approximately 6.04, 4.31 and 1.40. Based on the stability of the complex in saline, serum, in vitro binding with S. aureus and biodistribution results, the 99mTc–RUN complex is recommended for in vivo scintigraphic localization of the S. aureus in vivo infectious foci in human.

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Authors and Affiliations

  1. Nuclear Medicine Research Laboratory (NMRL), University of Peshawar, Peshawar, KPK, Pakistan

    Syed Qaiser Shah

  2. Phyotopharmaceutical and Neutraceuticals Research Laboratory (PNRL), University of Peshawar, Peshawar, KPK, Pakistan

    Muhammad Rafiullah Khan

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  1. Syed Qaiser Shah
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  2. Muhammad Rafiullah Khan
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Correspondence to Syed Qaiser Shah.

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Shah, S.Q., Khan, M.R. Radiocharacterization of the 99mTc–rufloxacin complex and biological evaluation in Staphylococcus aureus infected rat model. J Radioanal Nucl Chem 288, 373–378 (2011). https://doi.org/10.1007/s10967-010-0923-8

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  • DOI: https://doi.org/10.1007/s10967-010-0923-8

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