99mTc-labeling and molecular modeling of short dipeptide glycyl-l-proline
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Glycyl-l-proline (Gly-l-Pro) is the main degradation product of collagen and is a good diagnostic tool in various pathological conditions. The aim of this work was to prepare dipeptide Gly-l-Pro labeled with 99mTc. Complex preparation was carried out under alkaline reaction conditions and its stability was assessed 10 and 120 min after preparation. The formation of two types of complex compounds was observed. High-performance liquid chromatography, paper electrophoresis, paper chromatography and thin layer chromatography were employed to monitor the formation of different complexes. Molecular modeling (semi-empirical method) was used to design their structure and composition. First complex cI with formula [TcO(Gly-l-Pro)]−1 is unstable. After 120 min cI is completely transformed to complex cII with formula Tc(Gly-l-Pro)3.
Keywords99mTc Dipeptide Glycyl-l-proline Molecular modeling
This work was funded by grand from the Ministry of Education of the Slovak Republic number 1/0003/08.
- 8.Sassi ML (2001) Carboxyterminal degradation products of type I collagen. Oulu University Press, Oulu. ISBN 951-42-6491-6Google Scholar
- 9.Reiser KM, Amigable MA, Last JA (1992) J Biol Chem 267:24207–24216Google Scholar
- 15.Kotluv WL, Fried M, Gurd FRN (1959) J Am Chem Soc 82:233–241Google Scholar
- 24.Zolle I (2007) Technetium-99m pharmaceuticals. Springer, Berlin. ISBN-10-3-540-33989-2Google Scholar
- 26.Yoshihara K, Omori T (1996) Technetium and rhenium. Springer, Berlin. ISBN 3-540-59469-8Google Scholar
- 27.Hashimoto K, Kamal WHBBW, Matsuoka H (2005) J Nucl Radiochem Sci 6:193–196Google Scholar
- 33.Abram U, Abram S, Dilworth JR (1996) Acta Crystallogr C52:605–607Google Scholar