Radio labeling, quality control and biodistribution of 99mTc-cefotaxime as an infection imaging agent

  • S. F. Mirshojaei
  • M. Gandomkar
  • R. Najafi
  • S. E. Sadat Ebrahimi
  • M. H. Babaei
  • A. Shafiei
  • M. H. Talebi


Cefotaxime, a cephalosporin antibiotic, used to treat bacterial infections was investigated to label with 99mTc. Labeling was performed using sodium dithionite as a reducing agent at 100 °C for 10 min and radiochemical analysis involved ITLC and HPLC methods. The stability of labeled antibiotic was checked in the presence of human serum at 37 °C up to 24 h. The maximum radiolabeling yield was 92 ± 2%. Bacterial binding assay was performed with S. aureus and the in vivo distribution was studied in mice. Images showed minimal accumulation in non-target tissues, with an average target/non-target ratio of 2.89 ± 0.58.


99mTc Cefotaxime Radiolabeling Infection imaging 



The authors wish to thank Mr. Mirfallah and Mr. Mazidi of the radioisotope department (AEOI) for providing sodium pertechnetate and assistance in quality control tests.


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Copyright information

© Akadémiai Kiadó, Budapest, Hungary 2010

Authors and Affiliations

  • S. F. Mirshojaei
    • 1
  • M. Gandomkar
    • 2
  • R. Najafi
    • 2
  • S. E. Sadat Ebrahimi
    • 1
  • M. H. Babaei
    • 2
  • A. Shafiei
    • 1
  • M. H. Talebi
    • 2
  1. 1.Department of Medicinal Chemistry, Faculty of PharmacyTehran University of Medical ScienceTehranIran
  2. 2.Nuclear Science Research SchoolNuclear Science and Technology Research Institute (NSTRI), Atomic Energy Organization of IranTehranIran

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