Distribution of 131I-labeled recombinant human erythropoietin in maternal and fetal organs following intravenous administration in pregnant rats

  • O. Yilmaz
  • F. Y. Lambrecht
  • N. Gokmen
  • S. Erbayraktar
  • K. Durkan


The aim of the present study was to demonstrate the possible transplacental transmission of 131I labeled recombinant human erythropoietin (131I-rh-EPO) in pregnant rats and its distribution through maternal and fetal organs. Six Wistar Albino Rats in their pregnancy of 18 days were used 131I labeled recombinant human erythropoietin (specific activity = 2.4 μCi/IU) was injected into the tail vein of rats. After 30 minutes labeled erythropoietin infusion maternal stomach, kidney, lung, liver, brain and heart as well as fetus were removed. Then, the same organs were removed from each fetus. Measuring weight of maternal and fetal organs as well as placenta were followed by radioactivity count via Cd(Te) detector. 131I labeled recombinant human erythropoietin was found to be able to pass rat placenta and its distribution order in fetal organs was similar to those of maternal organs. Besides, as measurements were performed closer to cornu uteri, uptakes were decreasing in every fetus and its corresponding placenta.


Erythropoietin Recombinant Human Erythropoietin Label Compound Fetus Weight Radioactivity Count 
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  1. 1.
    J. W. Fisher, Exp. Biol. Med., 228 (2003) 1.Google Scholar
  2. 2.
    Z. Z. Chong, J. Q. Kang, K. Maiese, Curr. Drugs Targets-Cardiovasc. Haematol. Disord., 3 (2003) 141.CrossRefGoogle Scholar
  3. 3.
    H. Kinoshita, N. Ohishi, A. Okazaki, Arzneimittelforschung, 4 (1991) 568.Google Scholar
  4. 4.
    M. J. Koury, M. C. Bondurant, S. E. Graber, S. T. Sawyer, J. Clin. Invest., 82 (1988) 154.CrossRefGoogle Scholar
  5. 5.
    J. A. Widness, S. T. Sawyer, R. L. Schmidt, D. H. Chestnut, J. Dev. Physiol., 15 (1991) 139.Google Scholar
  6. 6.
    J. A. Widness, T. A. Malone, R. A. Mufson, Pediatr. Res., 25 (1989) 649.CrossRefGoogle Scholar
  7. 7.
    E. D. Zanjani, J. S. Pixley, N. Slotnick, F. R. Mackintosh, D. Ekhterae, G. Clemons, Pathobiology, 61 (1993) 211.CrossRefGoogle Scholar
  8. 8.
    H. Kinoshita, N. Ohishi, M. Kato, S. Tokura, A. Okazaki, Arzneim-Forsch/Drug Res., 42 (1992) 174.Google Scholar
  9. 9.
    E. C. Greene, Anatomy of the Rat, Circulatory System, 1st ed., Hafner Publishing Co., New York, 1963, p. 204.Google Scholar
  10. 10.
    J. A. Widness, R. L. Schmidt, S. T. Sawyer, J. Perinat. Med., 23 (1995) 61.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  • O. Yilmaz
    • 1
  • F. Y. Lambrecht
    • 2
  • N. Gokmen
    • 3
  • S. Erbayraktar
    • 4
  • K. Durkan
    • 2
  1. 1.Department of Animal Research CenterDokuz Eylul University School of MedicineIzmirTurkey
  2. 2.Department of Nuclear ApplicationsEge University Institute of Nuclear SciencesIzmirTurkey
  3. 3.Department of Anesthesiology and ReanimationDokuz Eylul University School of MedicineIzmirTurkey
  4. 4.Department of NeurosurgeryDokuz Eylul University School of MedicineIzmirTurkey

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