Advertisement

Passage rates in poultry digestion using stable isotope markers and INAA

  • C. S. Dunkley
  • W. -K. Kim
  • W. D. James
  • W. C. Ellis
  • J. L. McReynolds
  • L. F. Kubena
  • D. J. Nisbet
  • S. C. Ricke
Article

Abstract

A method has been developed for the study of passage rates and mean residence times (MRT) of test rations through the gastrointestinal tracts of layer hens. The use of rare earth elements as stable indigestible markers monitored by neutron activation analysis has been previously demonstrated in numerous species. In this study hafnium was used to mark corn and alfalfa rations as well as a combination ration made up of 90% alfalfa and 10% corn. The primary goal of the study was to evaluate the potential for use of rare earth stable markers in poultry digestion and to determine efficiency of meal marking, optimum exposure rates and determination limits for use in the design of future experimental protocols. Three groups of 10 hens each were fed a particular marked meal with fecal droppings monitored for 24 hours. The hens were sacrificed after a second dosed feeding and a delay of two or seven hours, and digesta was collected from each portion of the gastrointestinal tract. Fecal dry matter as well as digesta collected was then prepared for analysis and the elemental concentrations of hafnium were measured with instrumental neutron activation analysis (INAA). Element adsorption on alfalfa was far less efficient than on the corn ration, limiting the applicability of much of the alfalfa data to digestion studies. Passage rate curves were prepared for corn. The marker was found to primarily concentrate in the ileum at both sacrifice times.

Keywords

Instrumental Neutron Activation Analysis Hafnium Passage Rate Mean Residence Time Layer Ration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    B. Svihus, H. Hetland, M. Choct, F. Sundby, Br. Poult. Sci., 43 (2002) 662.CrossRefGoogle Scholar
  2. 2.
    D. E. Wakeling, The use of low calcium and low sodium diets to induce an egg production pause in commercial layers, in: Agric Dev. Advisory Service Bull., No. 8L-51-8-76, Starcross, Exeter, Devon, UK, 1978.Google Scholar
  3. 3.
    C. R. Douglas, R. H. Harms, H. R. Wilson, Poult. Sci., 51 (1972) 2015.Google Scholar
  4. 4.
    A. B. Gilbert, R. Blair, Br. Poult. Sci., 16 (1975) 547.CrossRefGoogle Scholar
  5. 5.
    C. C. Whitehead, D. W. F. Shannon, Br. Poult. Sci., 15 (1974) 429.CrossRefGoogle Scholar
  6. 6.
    W. G. Nesbeth, C. R. Douglas, R. H. Harms, Poult. Sci., 55 (1976) 2128.Google Scholar
  7. 7.
    J. T. Scott, C. R. Creger, Poult. Sci., 59 (1976) 2089.Google Scholar
  8. 8.
    R. L. Shippee, P. E. Stake, U. Koehn, J. L. Lambert, R. W. Simmons, Poult. Sci., 58 (1979) 949.Google Scholar
  9. 9.
    L. R. Arrignton, R. A. Santa Cruz, R. H. Harms, H. R. Wilson, J. Nutr., 92 (1967) 325.Google Scholar
  10. 10.
    K. L. Landers, C. Woodward, Z. R. Howard, S. G. Birkhold, S. C. Ricke, Bioresour. Technol., 96 (2005) 907.CrossRefGoogle Scholar
  11. 11.
    C. L. Woodward, Y. M. Kwon, L. F. Kubena, J. A. Byrd, R. W. Moore, D. J. Nisbet, S. C. Ricke, Poult. Sci., 84 (2005) 185.Google Scholar
  12. 12.
    R. Tukey, B. E. March, J. Biely, Poult. Sci., 37 (1958) 786.Google Scholar
  13. 13.
    J. D. van der Klis, A. van Vorst, Poult. Sci., 72 (1993) 503.Google Scholar
  14. 14.
    W. C. Ellis, H. Matis, T. M. Hill, M. R. Murphy, Methodology for estimating digestion and passage kinetics of forages, in: Forage Quality, Evaluation, Utilization, G. C. Fahey (Ed.), University of Nebraska, Lincoln, 1994, p. 682.Google Scholar
  15. 15.
    R. Worley, A. Clearfield, W. C. Ellis, Anim. Sci., 80 (2002) 3307.Google Scholar
  16. 16.
    W. D. James, F. F. Arnold, K. R. Pond, M. D. Glascock, T. G. Spalding, J. Radioanal. Nucl. Chem., 83 (1983) 209.Google Scholar
  17. 17.
    K. R. Pond, W. C. Ellis, W. D. James, A. G. Deswysen, J. Dairy Sci., 68 (1985) 745.CrossRefGoogle Scholar
  18. 18.
    M. J. Wylie, W. C. Ellis, J. H. Matis, E. M. Bailey, W. D. James, D. E. Beever, Brit. J. Nutrition, 83 (2000) 295.Google Scholar

Copyright information

© Springer Science+Business Media, LLC. 2007

Authors and Affiliations

  • C. S. Dunkley
    • 1
  • W. -K. Kim
    • 1
  • W. D. James
    • 2
  • W. C. Ellis
    • 3
  • J. L. McReynolds
    • 4
  • L. F. Kubena
    • 4
  • D. J. Nisbet
    • 4
  • S. C. Ricke
    • 1
  1. 1.Department of Poultry ScienceTexas A&M UniversityCollege StationUSA
  2. 2.Center for Chemical Characterization and Analysis, Department of ChemistryTexas A&M UniversityCollege StationUSA
  3. 3.Department of Animal ScienceTexas A&M UniversityCollege StationUSA
  4. 4.Southern Plains Research Center, U.S. Department of AgricultureAgricultural Research ServicesCollege StationUSA

Personalised recommendations