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Journal of Radioanalytical and Nuclear Chemistry

, Volume 269, Issue 2, pp 389–396 | Cite as

Assuring the reliability of mass spectrometry for the routine determination of traces of doping in horse-urine

  • I. M. H. Lima
  • M. N. Frota
  • E. A. N. Fernandes
  • P. Bode
  • F. S. Tagliaferro
Article

Summary

This paper describes an analytical method using a nuclear-related technique for the detection of forbidden doping substances in the urine of race horses. The proposed method, adapted from the Méthode Alcaline Sur C-18 developed by the French Laboratoire de Contrôle Antidopage, is based on gas chromatography separation followed by mass spectrometry (GC-MS). The method was validated for caffeine, identified as the most frequent doping substance in the Brazilian horseracing activity. This validation is also a major requirement to achieve ISO/IEC 17025 laboratory accreditation. The validation has led to several metrological challenges because the decisions are largely based on qualitative results (“false-positive” and/or “false-negative”) and the degree of accuracy, as well as the traceability had to be determined in the absence of certified matrix reference materials.

Keywords

Caffeine Nimesulide Dipyrone Theobromine Determination Limit 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag/Akadémiai Kiadó 2006

Authors and Affiliations

  • I. M. H. Lima
    • 1
  • M. N. Frota
    • 2
  • E. A. N. Fernandes
    • 3
  • P. Bode
    • 4
  • F. S. Tagliaferro
    • 5
  1. 1.Jockey Club Brasileiro
  2. 2.Pontifícia Universidade Católica
  3. 3.Centro de Energia Nuclear na Agricultura, University of S?o Paulo
  4. 4.Delft University of Technology, Reactor Institute Delft
  5. 5.Centro de Energia Nuclear na Agricultura, University of S?o Paulo

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