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Biocompatible tumor micro-environment responsive CS-g-PNIPAAm co-polymeric nanoparticles for targeted Oxaliplatin delivery

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Abstract

The objective of the research study was to develop and characterize a biodegradable, thermo and pH dual responsive Oxaliplatin-loaded chitosan-graft-poly-N-isopropylacrylamide (CS-g-PNIPAAm) co-polymeric nanoparticles as a tumor-targeting drug delivery system. CS-g-PNIPAAm co-polymers were synthesized, characterized and optimized its thermo and pH responsive properties for tumor microenvironment conditions. Optimized co-polymer could be efficiently loaded with Oxaliplatin in nanoparticle form, evaluated for their morphology (TEM), particle size, zeta potential, loading efficiency and drug content. In vitro drug release study at tumor microenvironment and physiological pH and temperature conditions. The in vitro drug release was optimal at above lower critical solution temperature (LCST) and tumor microenvironment pH when compared to physiological pH & temperature. MTT assay and fluorescence microscopic study showed that drug release and cell uptake was significantly enhanced in tumor microenvironment. In conclusion, the obtained nanoparticles appeared to be of great promise in tumor targeted drug delivery of oxaliplatin.

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Acknowledgements

Authors are thankful to the KLE University, Belagavi for provinding grant to perform this research work. Authors extend their regards to Dr. Prabhakar Kore Basic Science Research Centre, KLE University Belagavi for providing their amenities to carry out this work. The authors are also thankful to Central Institute of Fisheries Technology, Cochin, India for providing chitosan as gift sample.

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Authors and Affiliations

  1. Department of Pharmaceutics, KLE Academy of Higher Education and Research, KLE College of Pharmacy, Nehru Nagar, Belagavi, Karnataka, -590010, India

    Archana S. Patil & Anand P. Gadad

  2. Department of Pharmaceutical chemistry, K.L.E’s College of Pharmacy, Nippani, Karnataka, India

    Ravindra D. Hiremath

  3. Department of Pharmaceutical chemistry, S.E.T’s College of Pharmacy, Dharwad, Karnataka, India

    Shrinivas D. Joshi

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  1. Archana S. Patil
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Correspondence to Archana S. Patil.

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Patil, A.S., Gadad, A.P., Hiremath, R.D. et al. Biocompatible tumor micro-environment responsive CS-g-PNIPAAm co-polymeric nanoparticles for targeted Oxaliplatin delivery. J Polym Res 25, 77 (2018). https://doi.org/10.1007/s10965-018-1453-2

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