Waterborne polyurethane containing spiropyran groups(SPU) has been prepared by polycondensation of isophorone diisocyanate (IPDI), polytetrahydrofuran glycol (PTMG), dimethylol propionic acid (DMPA), 1,4-butanediol (BD), trimethylolpropane (TMP) and 1-(2-Hydroxyethyl)-3,3-dimethylindolino-6′-nitrobenzopyrylospiran(SP). Fourier transform infrared spectroscopy (FT-IR), Thermogravimetric analysis (TG), Scanning electron microscope (SEM) were utilized to characterize SPU films for their chemical structure, thermal stability, microscopic structure. The kinetics of photochromic behavior of SPU dispersion and film has also been investigated. The dispersion and film showed a very good photochromic effect. The decoloration rate of the SPU dispersion and film follow the kinetic of a first order reaction. The fatigue resistance of SPU film is better than that of its dispersion.