Journal of Polymer Research

, 21:550 | Cite as

In situ grafted nanostructured ZnO/carboxymethyl cellulose nanocomposites for efficient delivery of curcumin to cancer

  • Laxmi Upadhyaya
  • Jay Singh
  • Vishnu Agarwal
  • A.C. Pandey
  • Shiv P. Verma
  • Parimal Das
  • R. P. Tewari
Original Paper


In this present manuscript, zinc oxide (ZnO) nanoparticles embedded carboxymethyl cellulose (CMC) bionanocomposite were prepared by in situ grafting and the hydrophobic anticancer drug curcumin (Cur) was loaded into it. Structural, morphological, and physiochemical behavior of prepared curcumin-loaded CMC/ZnO nanocomposites (NCs) were characterized by FTIR, XRD, SEM, TEM, TGA, and DTA. The drug entrapment efficiency was evaluated and the in vitro efficacy as anticancer drug delivery vehicle was analyzed. The potential toxicity of curcumin-loaded ZnO/CMC NCs (Cur/ZnO/CMC NCs) was studied by using L929 and MA104 cell lines via MTT assay. The cellular uptake study of Cur/ZnO/CMC NCs by normal (L929) and cancer (MA104) cells carried out by using ethanol extraction and by FACS analysis has been reported. The results of this investigation demonstrate that the nanomatrix synthesized can effectively deliver the anticancer drug curcumin, and hence appears to be a promising nanoformulation for anticancer therapy and other biomedical applications.


Carboxymethyl cellulose ZnO nanoparticles Curcumin Cancer therapy 



We thank Prof. P. Chakrabarti, Director, Motilal Nehru National Institute of Technology, Allahabad, India for providing other necessary facilities for research work. The author LU kindly acknowledges the Ministry of Human Resource Development (MHRD), Govt. of India for providing senior research fellowship (SRF) for research. JS kindly acknowledges Department of Science and Technology, New Delhi, India for awarding the INSPIRE FACULTY AWARD [IFA-13-CH-105].


  1. 1.
    Di Sia P (2012) THz spectroscopy and nanostructures: a short interesting review. Lett Appl Nanobiosci 1:8Google Scholar
  2. 2.
    Jude IN, Luke MG, John K, Clement LH (2013) Development of novel chitosan-poly(N, N-diethylacrylamide) IPN films for potential wound dressing and biomedical applications. J Polym Res 20:1Google Scholar
  3. 3.
    Ionela A, Gheorghe F, Mariana C, Valeria H, Marieta C (2013) Thermo- and pH-sensitive interpenetrating poly(N-isopropylacrylamide)/carboxymethyl pullulan network for drug delivery. J Polym Res 20:293CrossRefGoogle Scholar
  4. 4.
    Xiu-Li W, Yan-Li Z, Dao-Lu T, Gui-Ying L, Yu-Zhong W (2012) Self-assembly, drug-delivery behavior, and cytotoxicity evaluation of amphiphilic chitosan-graft-poly(1,4-dioxan-2-one) copolymers. J Polym Res 19:1–9CrossRefGoogle Scholar
  5. 5.
    Shi L, Tang C, Yin C (2012) Glycyrrhizin-modified-O-carboxymethyl chitosan nanoparticles as drug vehicles targeting hepatocellular carcinoma. Biomaterials 33:7594CrossRefGoogle Scholar
  6. 6.
    Rejinold NS, Muthunarayanan M, Divyarani VV, Sreerekha PR, Chennazhi KP, Nair SV, Tamura H, Jayakumar R (2011) Curcumin loaded biocompatible thermoresponsive polymeric nanoparticles for cancer drug delivery. J Coll Interf Sci 360:39CrossRefGoogle Scholar
  7. 7.
    Jiugao Y, Jingwen Y, Baoxiang L, Xiaofei M (2009) Preparation and characterization of glycerol plasticized-pea starch/ZnO–carboxymethylcellulose sodium nanocomposites. Bioresour Technol 100:2832–41CrossRefGoogle Scholar
  8. 8.
    Bayarri S, Gonzalez-Tomas L, Costell L (2009) Viscoelastic properties of aqueous and milk systems with carboxymethyl cellulose. Food Hydrocoll 23:441CrossRefGoogle Scholar
  9. 9.
    Biswal DR, Singh RP (2004) Characterisation of carboxymethyl cellulose and polyacrylamide graft copolymer. Carbohydr Polym 57:379CrossRefGoogle Scholar
  10. 10.
    Pushpamalar V, Langford SJ, Ahmad M, Lim YY (2006) Optimization of reaction conditions for preparing carboxymethyl cellulose from sago waste. Carbohydr Polym 64:312–8CrossRefGoogle Scholar
  11. 11.
    Ueno T, Yokota S, Kitaoka T, Wariishi H (2007) Conformational changes in single carboxymethyl-cellulose chains on a highly oriented pyrolytic graphite surface under different salt conditions. Carbohydr Res 342:954–60CrossRefGoogle Scholar
  12. 12.
    Kuttan R, Bhanumathy P, Nirmala K, George MC (1985) Potential anticancer activity of turmeric (Curcuma longa). Cancer Lett 29:197CrossRefGoogle Scholar
  13. 13.
    Jordan WC, Drew CR (1996) Curcumin––a natural herb with anti- HIV activity. J Natl Med Assoc 88:33Google Scholar
  14. 14.
    Srimal RC, Dhawan BN (1973) Pharmacology of diferuloyl methane (curcumin), a non-steroidal anti-inflammatory agent. J Pharm Pharmacol 25:447CrossRefGoogle Scholar
  15. 15.
    Sharma OP (1976) Antioxidant activity of curcumin and related compounds. Biochem Pharmacol 25:1811CrossRefGoogle Scholar
  16. 16.
    Kim MK, Choi GJ, Lee HS (2003) Fungicidal property of Curcuma longa L. rhizome-derived curcumin against phytopathogenic fungi in a greenhouse. J Agric Food Chem 51:1578CrossRefGoogle Scholar
  17. 17.
    Tonnesen HH (2002) Solubility, chemical and photochemical stability of curcumin in surfactant solutions. Studies of curcumin and curcuminoids. Pharmazie 57:820Google Scholar
  18. 18.
    Yallapu MM, Jaggi M, Chauhan SC (2012) Curcumin nanoformulations: a future nanomedicine for cancer. Drug Discov Today 17:71CrossRefGoogle Scholar
  19. 19.
    Muqbil I, Masood A, Sarkar FH, Mohammad RM, Azmi AS: Progress in nanotechnology based approaches to enhance the potential of chemopreventive agents Cancers 3:428 (2011)Google Scholar
  20. 20.
    Yallapu MM, Jaggi M, Chauhan SC (2010) Poly(beta-cyclodextrin)/curcumin self-assembly: A novel approach to improve curcumin delivery and its therapeutic efficacy in prostate cancer cells. Macromol Biosci 10:1141CrossRefGoogle Scholar
  21. 21.
    Yallapu MM, Othman SF, Curtis ET, Gupta BK, Jaggi M, Chauhan SC (2011) Multi-functional magnetic nanoparticles for magnetic resonance imaging and cancer therapy. Biomaterials 32:1890CrossRefGoogle Scholar
  22. 22.
    Chandramouleeswaran S, TMhaske S, Kathe AA, Varadarajan PV, Prasad V, Vigneshwaran N (2007) Functional behaviour of polypropylene/ZnO–soluble starch nanocomposites. Nanotechnology 18:385702CrossRefGoogle Scholar
  23. 23.
    Zhou J, Xu N, Wang ZL (2006) Dissolving behavior and stability of Zno wires in biofluids: A study on biodegradability and biocompatibility. Adv Mater 18:2432CrossRefGoogle Scholar
  24. 24.
    Nie L, Gao L, Feng P, Zhang J, Fu X, Liu Y, Yan X, Wang T (2006) Three-dimensional functionalized tetrapod-like ZnO nanostructures for plasmid DNA delivery. Small 2:621CrossRefGoogle Scholar
  25. 25.
    Zhang LL, Jiang YH, Ding YL (2010) Mechanistic investigation into antibacterial behaviour of suspensions of ZnO nanoparticles against E. coli. J Nanopart Res 12:1625CrossRefGoogle Scholar
  26. 26.
    Huang ZB, Zheng X, Yan DH (2008) Toxicological effect of ZnO nanoparticles based on bacteria. Langmuir 24:4140CrossRefGoogle Scholar
  27. 27.
    Hanley C, Layne J, Punnoose A (2008) Preferential killing of cancer cells and activated human T cells using ZnO nanoparticles. Nanotechnology 19:295103CrossRefGoogle Scholar
  28. 28.
    Wang H, Wingett D, Engelhard MH (2009) Fluorescent dye encapsulated ZnO particles with cell- specific toxicity for potential use in biomedical applications. J Mater Sci Mater Med 20:11CrossRefGoogle Scholar
  29. 29.
    Rasmussen JW, Martinez E, Louka P, Wingett DG (2010) Zinc oxide nanoparticles for selective destruction of tumor cells and potential for drug delivery applications. Expert Opin Drug Deliv 7:1063CrossRefGoogle Scholar
  30. 30.
    Kim DG, Jeong YI, Choi C, Roh SH, Kang SK, Jang MK (2006) Retinol-encapsulated low molecular water-soluble chitosan nanoparticles. Int J Pharmaceut 319:130CrossRefGoogle Scholar
  31. 31.
    Das RK, Kasoju N, Bora U (2010) Encapsulation of curcumin in alginate-chitosan-pluronic composite nanoparticles for delivery to cancer cells Nanomed. Nanotechnol Biol Med 6:153CrossRefGoogle Scholar
  32. 32.
    Bisht S, Feldmann G, Soni S, Ravi R, Karikar C, Maitra M (2007) Polymeric nanoparticle-encapsulated curcumin (“nanocurcumin”): A novel strategy for human cancer therapy. J Nanobiotechnol 5:3CrossRefGoogle Scholar
  33. 33.
    Su JF, Huang Z, Yuan XY, Wang XY, Li M: Structure and properties of carboxymethyl cellulose/soy protein isolate blend edible films crosslinked by Maillard reactions Carbohydr Polym 79: 145–53 (2010)Google Scholar
  34. 34.
    Yallapua MM, Jaggi M, Chauhana SC: Cyclodextrin-curcumin selfassembly enhances curcumin delivery in prostate cancer cells Colloids Surfaces B 79:113 (2010)Google Scholar
  35. 35.
    Chen C, Yu B, Liu P, Liu JF, Wang L (2011) Investigation of nano-sized ZnO particles fabricated by various synthesis routes. J Ceramic Processing Res 12:420Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Laxmi Upadhyaya
    • 1
  • Jay Singh
    • 3
  • Vishnu Agarwal
    • 2
  • A.C. Pandey
    • 4
  • Shiv P. Verma
    • 5
  • Parimal Das
    • 5
  • R. P. Tewari
    • 1
  1. 1.Department of Applied MechanicsMotiLal Nehru National Institute of TechnologyAllahabadIndia
  2. 2.Department of BiotechnologyMotiLal Nehru National Institute of TechnologyAllahabadIndia
  3. 3.Department of Applied ChemistryDelhi Technological UniversityShahbadIndia
  4. 4.Nanotechnology Application CentreUniversity of AllahabadAllahabadIndia
  5. 5.Centre for Genetic Disorders, Faculty of ScienceBanaras Hindu UniversityVaranasiIndia

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