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Study on the preparation of genipin crosslinked chitosan microspheres of resveratrol and in vitro release

  • Yue Zhang
  • Yi-feng Yu
  • Xian-xian Shi
  • Shu-chun Zhao
  • Ai-bing Chen
  • Dao-wei Huang
  • Dong-jie Niu
  • Zhen Qin
Original Paper

Abstract

Resveratrol has attracted considerable public and scientific attention because of its beneficial effects on human health. In this study, resveratrol was encapsulated into chitosan microspheres for improving the stability and evaluating the ability of controlled release. Chitosan microspheres were prepared by using an emulsification-crosslinking method, and specifically, a natural extraction genipin was used as crosslinking agent for pursuit better physiological compatibility. Factors which influence on the shape and dispersity of microspheres were investigated to optimize the preparing process of resveratrol loaded genipin-chitosanmicrospheres. The optimum condition was obtained and the prepared resveratrol loaded genipin-chitosanmicrospheres were characterized by many technical instruments. Entrapment efficiency of resveratrol in genipin-chitosanmicrospheres is up to 87.6 %, and loading capacity is 7.9 %. The in vitro release rate in pH 3.6 buffer solution reached to 33.8 % within 72 h, but was lower in pH 7.4 buffer solution.

Keywords

Resveratrol Chitosan Genipin Microsphere In vitro release 

Notes

Acknowledgments

The authors would like to thank the mutual funds of Hebei Natural Science Foundation and CSPC Pharmaceutical Co., Ltd. (C2011208114). Supports for Hebei Research Center of Pharmaceutical and Chemical Engineering, Hebei Province Key Laboratory of Molecular Chemistry for Drug, and Innovation Team Funding of Hebei University of Science and Technology are also appreciated.

References

  1. 1.
    Robards K (2003) J Chromatogr A 1000:657–691CrossRefGoogle Scholar
  2. 2.
    Lucio M, Roberto MP, Tesei I (2006) J Chromatogr A 1114:263–268CrossRefGoogle Scholar
  3. 3.
    Claudine M, Gary W, Christine M (2005) J Clin Nutr 81:230S–242SGoogle Scholar
  4. 4.
    Frémont L (2000) Life Sci 66:663–673CrossRefGoogle Scholar
  5. 5.
    Gusman J, Malonne H, Atassi G (2001) Carcinogenesis 22:1111–1117CrossRefGoogle Scholar
  6. 6.
    Cai LS, Koziel JA, Dharmadhikari M (2009) J Chromatogr A 1216:281–287CrossRefGoogle Scholar
  7. 7.
    Juan ME, Alfaras I, Planas JM (2012) Pharmacol Res 65:584–591CrossRefGoogle Scholar
  8. 8.
    Siemann EH, Creasy LL (1992) Am J Enol Vitic 43:49–52Google Scholar
  9. 9.
    Soleas GJ, Diamandis EP, Goldberg DM (1997) Clin Biochem 30:91–113CrossRefGoogle Scholar
  10. 10.
    Luan TG, Li GK, Zhang ZX (2000) Anal Chim Acta 424:19–25CrossRefGoogle Scholar
  11. 11.
    Lucio M, Roberto P (2008) J Chromatogr A 1185:23–30CrossRefGoogle Scholar
  12. 12.
    Athar M, Back JH, Tang X (2007) Toxicol Appl Pharmacol 224:274CrossRefGoogle Scholar
  13. 13.
    Stocco B, Toledo K, Salvador M (2012) Maturitas 72:72–78CrossRefGoogle Scholar
  14. 14.
    Peng HL, Xiong H, Li JH (2010) Food Chem 121:23–28CrossRefGoogle Scholar
  15. 15.
    Kim YS, Sull JW, Sung HJ (2012) Mol Biol Rep 39:8709–8716CrossRefGoogle Scholar
  16. 16.
    Piotrowska H, Kucinska M, Murias M (2012) Mutat Res 750:60–82CrossRefGoogle Scholar
  17. 17.
    Timothy HS, Robert WM, Jr, Keith WH (2000) J Agric Food Chem 48:1243–1246Google Scholar
  18. 18.
    Clark D, Tuor UI, Thompson R (2012) PLoS One 7:e47792CrossRefGoogle Scholar
  19. 19.
    Goldberg DM (1995) Clin Chem 41:14–16Google Scholar
  20. 20.
    Zhang Y, Zhang YY, Yu CJ (2012) Adv Mater Res 479–481:833–836Google Scholar
  21. 21.
    Signorelli P, Ghidoni R (2005) J Nutr Biochem 16:449–466CrossRefGoogle Scholar
  22. 22.
    Lu Z, Cheng B, Hu YL (2009) Food Chem 113:17–20CrossRefGoogle Scholar
  23. 23.
    Juan ME, Buenafuente J, Casals I (2002) Food Res Int 35:195–199CrossRefGoogle Scholar
  24. 24.
    Babazadeh M, Edjlali L, Rashidian L (2007) J Polym Res 14:207–213CrossRefGoogle Scholar
  25. 25.
    Oh A, Yun JM, Kim H (2011) J Polym Res 18:2441–2447CrossRefGoogle Scholar
  26. 26.
    Vidgren P, Vidgren M, Arppe J (1992) Drug Dev Ind Pharm 18:581–597CrossRefGoogle Scholar
  27. 27.
    Anal AK, Stevens WF, Remuňán-López C (2006) Int J Pharm 312:166–173CrossRefGoogle Scholar
  28. 28.
    Gao JG, Zhang Y, Yu YF (2011) J Polym Res 18:1501–1508CrossRefGoogle Scholar
  29. 29.
    Liu ZY, Ge XJ, Lu Y (2012) Food Hydrocoll 26:311–317CrossRefGoogle Scholar
  30. 30.
    Chiu FC, Lai SM, Hsieh IC (2012) J Polym Res 19:9781–9785CrossRefGoogle Scholar
  31. 31.
    Casettari L, Vllasaliu D, Lam JKW (2012) Biomaterials 33:7565–7583CrossRefGoogle Scholar
  32. 32.
    Xu JH, Li SW, Tostado C (2009) Biomed Microdevices 11:243–249CrossRefGoogle Scholar
  33. 33.
    Ma LH, Liu CS (2010) Colloids Surf B Biointerfaces 75:448–453CrossRefGoogle Scholar
  34. 34.
    Zhang YL, Wei W, Lv PP (2011) Eur J Pharm Biopharm 77:11–19CrossRefGoogle Scholar
  35. 35.
    Zhang C, Cheng Y, Qu GW (2008) Carbohydr Polym 72:390–397CrossRefGoogle Scholar
  36. 36.
    Gupta KC, Jabrail FH (2006) Carbohydr Polym 66:43–54CrossRefGoogle Scholar
  37. 37.
    Kawadkar J, Chauhan MK (2012) Eur J Pharm Biopharm 81:563–572CrossRefGoogle Scholar
  38. 38.
    Harris R, Lecumberri E, Heras A (2010) Mar Drugs 8:1750–1762CrossRefGoogle Scholar
  39. 39.
    Karnchanajindanun J, Srisa-ard M, Baimark Y (2011) Carbohydr Polym 85:674–680CrossRefGoogle Scholar
  40. 40.
    Aldana AA, González A, Strumia MC (2012) Mater Chem Phys 134:317–324CrossRefGoogle Scholar
  41. 41.
    Chinese Pharmacopoeia (2010) The guiding principles for stability test of drugs and drug preparations: appendix XIX CGoogle Scholar
  42. 42.
    Chinese Pharmacopoeia (2010) The guiding principles for sustained release, controlled release and delayed released of Pharmaceutical preparations: appendix XIX DGoogle Scholar
  43. 43.
    Chen XL, Zhao GW, Liao ZG (2010) Proceedings of academic development BBS of Chinese medicine in Jiangxi Province: 273–279Google Scholar
  44. 44.
    Agnihotri SA, Mallikarjuna NN, Aminabhavi TM (2004) J Control Release 100:5–28CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Yue Zhang
    • 1
    • 2
    • 3
  • Yi-feng Yu
    • 1
    • 2
    • 3
  • Xian-xian Shi
    • 1
  • Shu-chun Zhao
    • 1
    • 2
    • 3
  • Ai-bing Chen
    • 1
    • 2
  • Dao-wei Huang
    • 1
  • Dong-jie Niu
    • 1
  • Zhen Qin
    • 1
  1. 1.School of Chemical and Pharmaceutical EngineeringHebei University of Science and TechnologyShijiazhuangChina
  2. 2.Hebei Research Center of Pharmaceutical and Chemical EngineeringShijiazhuangChina
  3. 3.State Key Laboratory Breeding Base-Hebei Province Key Laboratory of Molecular Chemistry for DrugShijiazhuangChina

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