Self-assembly, drug-delivery behavior, and cytotoxicity evaluation of amphiphilic chitosan-graft-poly(1,4-dioxan-2-one) copolymers
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A series of biocompatible and amphiphilic chitosan-graft-poly(1,4-dioxan-2-one) (CS-g-PPDO) copolymers were synthesized using a low molecular weight chitosan (M n = 5 × 103 Da). The resulting copolymers could self-assemble into micelles in aqueous medium. The critical micelle concentration, size, and morphology of the micelles were characterized by fluorescence spectra, dynamic light scattering (DLS), atomic force microscopy (AFM), and transmission electron microscopy (TEM). These polymer micelles were used as drug carriers for nimodipine (a model drug). It was found that nimodipine was released from the micelles for periods as long as 100 h without a burst release occurring. In addition, the MTT assay showed that the CS-g-PPDO copolymers exhibited low cytotoxicity and good biocompatibility with L929 cells, highlighting the potential of these copolymers as drug-delivery carriers.
KeywordsChitosan Poly(1,4-dioxan-2-one) Micelle Drug release Cytotoxicity
This work was supported financially by the National Science Foundation of China (20974066), the Excellent Youth Foundation of Sichuan (2011JQ0007), the Science Foundation for The Excellent Youth Scholars of Sichuan University (2011SCU04B32), the Program of International S & T Cooperation (2011DFA51420), and the Program for Changjiang Scholars and Innovative Research Team in the University of the Ministry of Education of China (IRT1026). The authors also acknowledge the West China College of Stomatology (Sichuan University) for the MTT assay.
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