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Conformation of ATP and ADP Molecules in Aqueous Solutions Determined by High-Energy X-ray Diffraction

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Abstract

High-energy X-ray diffraction measurements were carried out at 26 °C for aqueous 1.0, 2.0 and 2.05 mol% disodium adenosine 5′-triphosphate (ATP) and 2.0 and 2.05 mol% disodium adenosine 5′-diphosphate (ADP) solutions in order to obtain direct experimental information on the intramolecular conformations of ATP and ADP molecules in aqueous solutions. Observed interference terms were analyzed in terms of the intramolecular geometry of the ATP and ADP molecules. Dihedral angles between adenine and the ribose group (t 1), ribose-ring and methylene group of ribose (t 2), and the methylene group of ribose and triphosphate (or diphosphate) group (t 3), were determined through the least-squares fitting procedure of the observed interference term.

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Acknowledgments

The authors would like to thank Dr. Shinji Kohara (Japan Synchrotron Research Institute) for his help during X-ray diffraction measurements. The synchrotron radiation experiments were performed with the approval of JASRI (Proposal Nos. 2011A1368 and 2012B1509).

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Authors and Affiliations

  1. Department of Material and Biological Chemistry, Faculty of Science, Yamagata University, Yamagata, Yamagata, 990-8560, Japan

    Takuya Miyazaki, Yasuo Kameda, Yuko Amo & Takeshi Usuki

  2. Graduate School of Science and Technology, Niigata University, Niigata, 950-2181, Japan

    Yasuhiro Umebayashi & Hiroyuki Doi

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  1. Takuya Miyazaki
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  2. Yasuo Kameda
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  3. Yasuhiro Umebayashi
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  4. Hiroyuki Doi
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  5. Yuko Amo
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  6. Takeshi Usuki
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Correspondence to Yasuo Kameda.

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Miyazaki, T., Kameda, Y., Umebayashi, Y. et al. Conformation of ATP and ADP Molecules in Aqueous Solutions Determined by High-Energy X-ray Diffraction. J Solution Chem 43, 1487–1498 (2014). https://doi.org/10.1007/s10953-014-0153-8

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