Journal of Structural Chemistry

, Volume 48, Issue 4, pp 729–733 | Cite as

DNA packaging in water solutions induced by polylysine and spermidine

  • N. A. Kas’yanenko
  • B. A. Dribinskii


The conformation properties of the DNA molecule complexed with polylysine (PLL) and spermidine (Spd) were studied by circular dichroism (CD), ultraviolet spectrophotometry, dynamic light scattering (DLS), viscosimetry, and dynamic birefringence. Experimental data suggest that when the concentration ratio of the monomer residues of polylysine and DNA (N/P) in moles is smaller than unity, the viscosity changes but slightly, whereas when N/P > 1, it abruptly drops to solvent viscosity. This is accompanied by changes in optical anisotropy and in the circular dichroism spectra of DNA. DLS data indicate that for N/P > 1, compact discrete particles are formed.


DNA packaging polylysine spermidine 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    H. Eliyahu, Y. Barenholz, and A. J. Domb, Molecules, 10, 34–64 (2005).CrossRefGoogle Scholar
  2. 2.
    P. Dubruel and E. Schacht, Macromol. Biosci., 6, 789–810 (2006).CrossRefGoogle Scholar
  3. 3.
    N. A. Kas’yanenko, A. M. Kopyshev, O. N. Obukhova, et al., Zh. Fiz. Khim., 76, 2021–2026 (2002).Google Scholar
  4. 4.
    A. V. Slita, N. A. Kas’yanenko, O. V. Nazarova, et al., J. Biotechnol., 127, No. 4, 679–693 (2007).CrossRefGoogle Scholar
  5. 5.
    S. D. Patil, D. G. Rhodes, and D. J. Burgess, AAPS J., 7, No. 1, E61–E77 (2005).CrossRefGoogle Scholar
  6. 6.
    C. P. Lollo, M. G. Banaszczyk, P. M. Mullen, et al., Meth. Mol. Med., 69, 1–13 (2002).Google Scholar
  7. 7.
    L. C. Gosule and J. A. Schellman, Nature, 259, 333–335 (1976).CrossRefGoogle Scholar
  8. 8.
    H. G. Hansma, L. I. Pietrasanta, R. Golan, et al., J. Biomol. Struct. Dyn., 2, 271–275 (2000).Google Scholar
  9. 9.
    V. A. Bloomfield, Curr. Opin. Struct. Biol., 6, 334–341 (1996).CrossRefGoogle Scholar
  10. 10.
    N. V. Hud and K. H. Downing, Proc. Natl. Acad. Sci. USA, 98, 14925–14930 (2001).Google Scholar
  11. 11.
    É. V. Frisman, L. V. Shchagina, and V. I. Vorob’yov, Biorheol., 2, 189–194 (1965).Google Scholar
  12. 12.
    É. V. Frisman, M. A. Sibileva, and A. V. Krasnoperova, Vysokomolek. Soedin., 1, 597–606 (1962).Google Scholar

Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  1. 1.Physical FacultySt. Petersburg State UniversitySt. Petersburg

Personalised recommendations