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Journal of Porous Materials

, Volume 19, Issue 5, pp 587–595 | Cite as

In situ synthesis of monolithic molecularly imprinted stationary phases for liquid chromatographic enantioseparation of dibenzoyl tartaric acid enantiomers

  • Xiaoqing Chen
  • Weijie Yang
  • Yangmeihui Zhou
  • Feipeng Jiao
Article

Abstract

A monolithic molecularly imprinted polymer (monolithic MIP) for dibenzoyl-D-tartaric acid (D-DBTA) was prepared in a stainless-steel chromatographic column tube (50 mm × 4.6 mm I.D.) as HPLC stationary phase through in situ polymerization. By optimizing polymeric and chromatographic conditions, the chiral separation of DBTA enantiomers was successfully achieved in the obtained MIP in less than 25 min with a resolution Rs = 1.25, whereas no enantioseparation effect was found on the monolithic non-imprinted polymer (NIP). Thermodynamic data of the enantioseparation were calculated. The results revealed that two different thermodynamic processes existed within the temperature range investigated, moreover, just at the transition temperature (50 °C) of the two processes, separation factor α reached its maximum. Scathcard analysis indicated that only one class of binding sites existed in the obtained MIP, with its K d and Q max estimated to be 5.457 × 10−4 mol L−1 and 229.6 μmol g−1, respectively. Nitrogen adsorption experiment proved that the prepared MIP had a large specific surface area of 105 m2 g−1. Scanning electron microscopy showed that large flow-through pores were present in the prepared monolith. As a consequence, the column backpressure was only 1.2 MPa with acetonitrile as mobile phase at a flow rate of 1.0 mL min−1.

Keywords

DBTA Enantioseparation In situ synthesis Monolithic column Molecularly imprinted polymer 

Notes

Acknowledgments

We acknowledge the supports given to this work by the China National Natural Science Foundation (Project No. 20805058) and Hunan Provincial Postdoctoral Special Foundation of China (Project No. 2009RS3039) and Hunan Provincial Natural Science Foundation of China (Project No. 09JJ3026) and Undergraduate Innovational Experimentation Program of Central South University (LB10055).

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Xiaoqing Chen
    • 1
  • Weijie Yang
    • 1
  • Yangmeihui Zhou
    • 1
  • Feipeng Jiao
    • 1
  1. 1.School of Chemistry and Chemical EngineeringCentral South UniversityChangshaChina

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