A method to regenerate the damaged Chironomidae slides in entellan mounting solution
This paper describes a new method to regenerate damaged Chironomidae head capsule slides mounted in entellan mounting solution. Many paleolimnologists use entellan mounting medium to mount subfossil chironomid head capsules for their routine investigations. These slides may get damaged or head capsules may be obscured by air bubble because they were not properly mounted. This makes the identification difficult and therefore, recovering the damaged slides would be a necessary task for them. Using boiling water we separated cover slips from the slides and using 100 % 2-propanol we dissolved the entellan and recovered the mounted head capsules in the entellan mounting solution. Head capsules recovered in this procedure can be remounted.
KeywordsEntellan Chironomidae Head capsule Slide Mounted Restoration
Many thanks to Kristie Heard and David Hryn of National Water Institute, University of New Brunswick, Fredericton.
- Andersen T, Cranston PS, Epler JH (2013) Chironomidae of the Holarctic region: keys and diagnosis—Larvae. Insect Syst Evol 66:1–573Google Scholar
- Epler H (2001) Identification manual for the larval Chironomidae (Diptera) of North and South Carolina. North Carolina Department of Environment and Natural Resources Division of Water QualityGoogle Scholar
- Kobayashi T (2013) Regeneration of old slide specimens mounted in Hoyer’s medium. Chironomus Newsl Chiron Res 26:52Google Scholar
- Walker IR (2007) The WWW field guide to fossil midges. http://www.paleolab.ca/wwwguide/index.html