The Protein Journal

, Volume 35, Issue 3, pp 231–236 | Cite as

Expression Profiles of IGF-1R Gene and Polymorphisms of its Regulatory Regions in Different Pig Breeds

  • Yunyun Cheng
  • Songcai Liu
  • Xin Zhang
  • Qingyan Wu
  • Siming Li
  • Haoyu Fu
  • Lijie Dong
  • Hao Yu
  • Linlin Hao


Insulin like growth factor 1 receptor (IGF-1R) is a candidate gene for growth and carcass traits in regulating animal growth, metabolism and endocrine. It is widely expressed in liver, muscle, bone tissues where the IGF-1R functions as a factor that promotes cell growth. In this study, the protein expression level of IGF-1R gene in liver and muscle tissues of three periods (birth, weaning and adult) of three pig breeds (BamaXiang pigs (BM), Tibetan pigs (TM) and Junmu No.1 pigs (JM)) were tested by western blot. SNPs within the regulatory region of pig IGF-1R gene were detected using direct sequencing and then the genotypes were identified through AS-PCR approach. Results showed expression profiles of IGF-1R gene between liver and muscle tissues were different and significant differences were also found among pig breeds. In the same time, four SNPs were detected in the regulatory region of IGF-1R gene, among which the genotype frequency of three (g.−1468G > C, g.−1192 C > T and g.330,424 C > T) were significantly different among the pig breeds. BM tended to heterozygous (GC/CT) of the anterior two loci, while TM and JM preferred the other two homozygotes respectively. For the g.330,424 C > T, all pig breeds were tended to be the heterozygous. In conclusion, the SNPs with different genotype distribution among the three pig breeds may explain the gene expression difference between the different pig breeds.


IGF-1R gene Tissue expression Regulatory region Polymorphisms Pigs 



Insulin like growth factor 1 receptor





This work was supported by the National Natural Science Foundation of China (31101781, 31072102) and the Programs Foundation of Ministry of Education of China (20110061110081).


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Yunyun Cheng
    • 1
  • Songcai Liu
    • 1
    • 2
  • Xin Zhang
    • 3
  • Qingyan Wu
    • 1
  • Siming Li
    • 1
  • Haoyu Fu
    • 1
  • Lijie Dong
    • 1
  • Hao Yu
    • 1
  • Linlin Hao
    • 1
  1. 1.College of Animal ScienceJilin UniversityChangchunChina
  2. 2.Five-Star Animal Health Pharmaceutical Factory of Jilin ProvinceChangchunChina
  3. 3.China-Japan Union HospitalJilin UniversityChangchunChina

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