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The Protein Journal

, Volume 35, Issue 3, pp 218–224 | Cite as

Effect of Cadmium Ion on alpha-Glucosidase: An Inhibition Kinetics and Molecular Dynamics Simulation Integration Study

  • Tao Luo
  • Jinhyuk Lee
  • Zhi-Rong Lü
  • Hang Mu
  • Li-Mei Yue
  • Yong-Doo Park
  • Zhuo-Ming Ye
Article

Abstract

α-Glucosidase is a critical metabolic enzyme that produces glucose molecules by catalyzing carbohydrates. The aim of this study is to elucidate biological toxicity of Cd2+ based on α-glucosidase activity and conformational changes. We studied Cd2+-mediated inactivation as well as conformational modulation of α-glucosidase by using kinetics coupled with simulation of molecular dynamics. The enzyme was significantly inactivated by Cd2+ in a reversibly binding behavior, and Cd2+ binding induced a non-competitive type of inhibition reaction (the K i was calculated as 0.3863 ± 0.033 mM). Cd2+ also modulated regional denaturation of the active site pocket as well as overall partial tertiary structural change. In computational simulations using molecular dynamics, simulated introduction of Cd2+ induced in a depletion of secondary structure by docking Cd2+ near the saccharides degradation at the active site, suggesting that Cd2+ modulating enzyme denaturation. The present study elucidated that the binding of Cd2+ triggers conformational changes of α-glucosidase as well as inactivates catalytic function, and thus suggests an explanation of the deleterious effects of Cd2+ on α-glucosidase.

Keywords

α-Glucosidase Cd2+ Inhibition kinetics Denaturation Molecular dynamics 

Abbreviations

pNPG

p-Nitrophenyl α-d-glucopyranoside

pNP

4-Nitrophenol

ANS

1-Anilino-8-naphthalenesulfonate

MD

Molecular dynamics

Notes

Acknowledgments

Dr. Hang Mu was supported by a grant from the Science and Technology Planning Project of Jiaxing (No. 2013AY21032). Dr. Jinhyuk Lee was supported through grants from the KOBIC Research Support Program, KRIBB Research Initiative Program, and the Pioneer Research Center Program through the National Research Foundation of Korea funded through the Ministry of Science, ICT & Future Planning (2013M3C1A3064780). Dr. Yong-Doo Park was supported through a grant from the Zhejiang Provincial Natural Science Foundation of China, “Towards studying the function of C3dg protein and elucidating its role in the pathogenesis of atopic dermatitis” (Grant No. LY14H110001) and a fund from the Science and Technology Planning Project of Jiaxing (No. 2014AY21026).

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Tao Luo
    • 1
  • Jinhyuk Lee
    • 2
    • 3
  • Zhi-Rong Lü
    • 4
  • Hang Mu
    • 4
  • Li-Mei Yue
    • 1
  • Yong-Doo Park
    • 4
  • Zhuo-Ming Ye
    • 1
  1. 1.Department of Epidemiology, School of Public Health and Tropical Medicine, Guangdong Provincial Key Laboratory of Tropical Disease ResearchSouthern Medical UniversityGuangzhouPeople’s Republic of China
  2. 2.Korean Bioinformation Center (KOBIC)Korea Research Institute of Bioscience and BiotechnologyDaejeonKorea
  3. 3.Department of Nanobiotechnology and BioinformaticsUniversity of Sciences and TechnologyDaejeonKorea
  4. 4.Zhejiang Provincial Key Laboratory of Applied EnzymologyYangtze Delta Region Institute of Tsinghua UniversityJiaxingPeople’s Republic of China

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