The Protein Journal

, Volume 31, Issue 3, pp 206–211 | Cite as

Isolation of α-Glucuronidase Enzyme from a Rumen Metagenomic Library

  • Charles C. Lee
  • Rena E. Kibblewhite
  • Kurt Wagschal
  • Ruiping Li
  • William J. Orts


α-Glucuronidase enzymes play an essential role in the full enzymatic hydrolysis of hemicellulose. Up to this point, all genes encoding α-glucuronidase enzymes have been cloned from individual, pure culture strains. Using a high-throughput screening strategy, we have isolated the first α-glucuronidase gene (rum630-AG) from a mixed population of microorganisms. The gene was subcloned into a prokaryotic vector, and the enzyme was overexpressed and biochemically characterized. The RUM630-AG enzyme had optimum activity at pH 6.5 and 40 °C. When birchwood xylan was used as substrate, the RUM630-AG functioned synergistically with an endoxylanase enzyme to hydrolyze the substrate.


α-Glucuronidase Rumen Metagenome Library screening Hemicellulose 



4-O-methyl-d-glucuronic acid


Glycosyl hydrolase


Open reading frame


α-Glucuronidase from rumen microorganism



Mention of trade names or commercial products in this report is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer.


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Copyright information

© Springer Science+Business Media, LLC (outside the USA)  2012

Authors and Affiliations

  • Charles C. Lee
    • 1
  • Rena E. Kibblewhite
    • 1
  • Kurt Wagschal
    • 1
  • Ruiping Li
    • 2
  • William J. Orts
    • 1
  2. 2.Engineering Research Center of Eco-Environment in Three Gorges Reservoir RegionMinistry of Education, China Three Gorges UniversityYichangChina

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