The Protein Journal

, Volume 31, Issue 2, pp 150–157 | Cite as

Human Serum Protein Adsorption onto Synthesis Nano-Hydroxyapatite

  • M. Mohsen-Nia
  • M. Massah Bidgoli
  • M. Behrashi
  • A. Mohsen Nia


Adsorption of human serum proteins (Albumin and total protein) onto high purity synthesis nano-hydroxyapatite (HA), Ca10(PO4)6(OH)2, has been studied in a wide temperature range by UV–visible spectrophotometer. Adsorption isotherm is basically important to describe how solutes interact with adsorbent, and is critical in optimizing the use of adsorbent. In the present study, the experimental results were fitted to the Langmuir, Freundlich, Temkin and Dubinin-Radushkevich (DR) models to obtain the characteristic parameters of each model and square of the correlation coefficients (R2). According to the results, the DR isotherm model had the best agreement with the experimental data. The effect of temperature on adsorption of human serum proteins (HSP) onto the synthesized nano-HA was studied. The experimental results indicated that temperature increase generally causes an increase in the adsorption of HSP onto the nano-HA. This is basically due to the effect of temperature on the HSP activity and its diffusion rate on HA surfaces.


Adsorption isotherm Albumin Human serum protein UV–Visible Nano-hydroxyapatite 







Square of the correlation coefficients


Human serum proteins




Bromocresol Green


Adsorption capacity


Equilibrium concentration


Initial protein concentration


Volume of solution containing protein


Mass of nano-HA adsorbent


Maximum amount of the adsorbed protein per unit weight of adsorbent


Langmuir equilibrium constant


Dimensionless equilibrium constant


Freundlich constants


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • M. Mohsen-Nia
    • 1
    • 2
  • M. Massah Bidgoli
    • 1
  • M. Behrashi
    • 3
  • A. Mohsen Nia
    • 4
  1. 1.Department of ChemistryUniversity of KashanKashanIran
  2. 2.Division of Chemistry and Chemical EngineeringCaltechPasadenaUSA
  3. 3.Kashan University of Medical SciencesKashanIran
  4. 4.Department of Computer EngineeringSharif University of TechnologyTehranIran

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