Advertisement

The Protein Journal

, Volume 31, Issue 2, pp 150–157 | Cite as

Human Serum Protein Adsorption onto Synthesis Nano-Hydroxyapatite

  • M. Mohsen-Nia
  • M. Massah Bidgoli
  • M. Behrashi
  • A. Mohsen Nia
Article

Abstract

Adsorption of human serum proteins (Albumin and total protein) onto high purity synthesis nano-hydroxyapatite (HA), Ca10(PO4)6(OH)2, has been studied in a wide temperature range by UV–visible spectrophotometer. Adsorption isotherm is basically important to describe how solutes interact with adsorbent, and is critical in optimizing the use of adsorbent. In the present study, the experimental results were fitted to the Langmuir, Freundlich, Temkin and Dubinin-Radushkevich (DR) models to obtain the characteristic parameters of each model and square of the correlation coefficients (R2). According to the results, the DR isotherm model had the best agreement with the experimental data. The effect of temperature on adsorption of human serum proteins (HSP) onto the synthesized nano-HA was studied. The experimental results indicated that temperature increase generally causes an increase in the adsorption of HSP onto the nano-HA. This is basically due to the effect of temperature on the HSP activity and its diffusion rate on HA surfaces.

Keywords

Adsorption isotherm Albumin Human serum protein UV–Visible Nano-hydroxyapatite 

Abbreviations

HA

Hydroxyapatite

DR

Dubinin-Radushkevich

R2

Square of the correlation coefficients

HSP

Human serum proteins

R

Reagent

BCG

Bromocresol Green

qe

Adsorption capacity

Ce

Equilibrium concentration

C0

Initial protein concentration

V

Volume of solution containing protein

M

Mass of nano-HA adsorbent

qm

Maximum amount of the adsorbed protein per unit weight of adsorbent

Kl

Langmuir equilibrium constant

Rl

Dimensionless equilibrium constant

Kf,n

Freundlich constants

References

  1. 1.
    Arai T, Norde W (1990) Colloids Surf 51:1–15CrossRefGoogle Scholar
  2. 2.
    Arai T, Norde W (1990) Colloids Surf 51:17–28CrossRefGoogle Scholar
  3. 3.
    Bremer MGEG, Duval J, Norde W, Lyklema J (2004) Colloids Surf A Physicochem Eng Aspects 250:29–42CrossRefGoogle Scholar
  4. 4.
    Burtis CA, Ashwood ER, Bruns DE (2008) Tietz Fundamentals of Clinical Chemistry, 6th edn. Elsevier, St Louis: SaundersGoogle Scholar
  5. 5.
    Doumas BT, Ard Watson W, Biggs HG (1971) Clin Chim Acta 31:87–96CrossRefGoogle Scholar
  6. 6.
    Dubinin MM (1975) Progress in surface and membrane science. In: Cadenhead DA et al. (Eds.), Academic Press, New YorkGoogle Scholar
  7. 7.
    Elgersma AV, Zsom RLJ, Lyklema J, Norde W (1992) Colloids Surf 65:17–28CrossRefGoogle Scholar
  8. 8.
    Elliott J (1994) Structure and chemistry of the apatites and other calcium orthophosphates. Elsevier, New YorkGoogle Scholar
  9. 9.
    Falch DK (1981) J Clin Lab Invest 41:59–62CrossRefGoogle Scholar
  10. 10.
    Faulhaber I, Bernardi G (1967) Biochim Biophys Acta 140:561–564Google Scholar
  11. 11.
    Freundlich HMF (1906) J Phys Chem 57:385–470Google Scholar
  12. 12.
    Gorbunoff MJ (1984) Anal Biochem 136:425–432CrossRefGoogle Scholar
  13. 13.
    Gorbunoff MJ (1984) Anal Biochem 136:433–439CrossRefGoogle Scholar
  14. 14.
    Gorbunoff MJ, Timasheff SN (1984) Anal Biochem 136:440–445CrossRefGoogle Scholar
  15. 15.
    Hall KR, Eagleton LC, Acrivos A, Vermeulen T (1966) Ind Eng Chem Fundam 5:212–223CrossRefGoogle Scholar
  16. 16.
    Ho YS, Huang CT, Huang HW (2002) Process Biochem 37:1421–1430CrossRefGoogle Scholar
  17. 17.
    Hutson ND, Yang RT (1997) Adsorption 3:189–195CrossRefGoogle Scholar
  18. 18.
    Kawasaki T, Kobayashi W, Ikeda K, Takahashi S, Monma H (1986) Eur J Biochem 157:291–295CrossRefGoogle Scholar
  19. 19.
    Kawasaki T, Ikeda K, Takahashi S, Kuboki Y (1986) Eur J Biochem 155:249–257CrossRefGoogle Scholar
  20. 20.
    Kondo A, Mihara J (1996) Colloids Surf 177:214–221CrossRefGoogle Scholar
  21. 21.
    Kopac T, Bozgeyik K, Yener J (2008) Colloids Surf A Physicochem Eng Aspects 322:19–28CrossRefGoogle Scholar
  22. 22.
    Kupke IR, Kather B, Zeugner S (1981) Clin Chim Acta 112:177–185CrossRefGoogle Scholar
  23. 23.
    Langmuir I (1918) J Am Chem Soc 40:1361–1403CrossRefGoogle Scholar
  24. 24.
    Luo Q, Andrade JD (1998) Colloids Surf 200:104–113CrossRefGoogle Scholar
  25. 25.
    Mohsen-Nia M, Massah Bidgoli M, Submitted paperGoogle Scholar
  26. 26.
    Mori O, Imae T (1997) Colloids Surf B Biointerf 9:31–36CrossRefGoogle Scholar
  27. 27.
    Norde W (1986) Adv Colloids Interface Sci 25:267–340CrossRefGoogle Scholar
  28. 28.
    Peters T (1996) All about albumin; biochemistry, genetics, and medical applications. Academic Press, San DiegoGoogle Scholar
  29. 29.
    Rengaraj S, Kim Y, Joo CK, Choi K, Yi J (2004) Korean J Chem Eng 21:187–194CrossRefGoogle Scholar
  30. 30.
    Rodkey FL (1965) Clin Chem 11:478–487Google Scholar
  31. 31.
    Schaller J, Gerber S, Kampfer U, Lejon S, Trachsel C (2008) Human blood plasma proteins: structure and function. Wiley, EnglandCrossRefGoogle Scholar
  32. 32.
    Shi QH, Tian Y, Dong XY, Bai S, Sun Y (2003) Biochem Eng J 16:317–322CrossRefGoogle Scholar
  33. 33.
    Sakiyama T, Tomura J, Imamura K, Nakanishi K (2004) Colloids Surf B Biointerf 33:77–84CrossRefGoogle Scholar
  34. 34.
    Temkin MI, Pyzhev V (1940) Acta Physiochim URSS 12:327–356Google Scholar
  35. 35.
    Yin G, Liu Z, Zhan J, Ding F, Yuan N (2002) Chem Eng J 87:181–186CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • M. Mohsen-Nia
    • 1
    • 2
  • M. Massah Bidgoli
    • 1
  • M. Behrashi
    • 3
  • A. Mohsen Nia
    • 4
  1. 1.Department of ChemistryUniversity of KashanKashanIran
  2. 2.Division of Chemistry and Chemical EngineeringCaltechPasadenaUSA
  3. 3.Kashan University of Medical SciencesKashanIran
  4. 4.Department of Computer EngineeringSharif University of TechnologyTehranIran

Personalised recommendations