The Protein Journal

, Volume 30, Issue 1, pp 39–51 | Cite as

Purification, Characterization, Sequencing and Biological Chemistry of Galectin-1 Purified from Capra hircus (goat) Heart

  • Ghulam Md Ashraf
  • Naheed Banu
  • Ausaf Ahmad
  • Lamabam Peter Singh
  • Rajnish Kumar


A soluble β-galactoside binding 14.5 kDa lectin was purified from the heart of Capra hircus. Its metal independent nature, preferential affinity for β-d-lactose and 90–94% homology with carbohydrate recognition domain of previously reported galectin-1 confirmed its inclusion in galectin-1 subfamily. The secondary structures of the deduced amino acid sequences were generally conserved with previously reported Gal-1. Exposure of the purified protein to varying temperature and pH, oxidant, thiol blocking reagents, denaturants and detergents resulted in significant changes in UV (ultraviolet), fluorescence, CD (circular dichroism) and FTIR (fourier transform infra red) spectra, thus strongly emphasizing the vitality of regular secondary structure of galectins for maintaining their active conformation. Bioinformatics studies corroborated the results obtained in wet lab. Our findings based on physico-chemical properties, oxidative inactivation and structural analysis of the goat heart galectin-1 suggests significant implications in potential biological and clinical applications.


Goat heart galectin-1 (GHG-1) Capra hircus Purification Cardiovascular disorders Oxidation Sequencing 3D JIGSAW 





Circular dichroism


Coomassie brilliant blue


Fourier transform infra red




High performance liquid chromatography


Sodium dodecyl sulphate polyacrylamide gel electrophoresis


Ultraviolet visible


Goat heart galectin-1


Phosphate buffered saline


Carbohydrate recognition domain



The financial assistance in the form of scholarships to the first author (GMA) from University Grants Commission (UGC), New Delhi is thankfully acknowledged. The authors are grateful to Aligarh Muslim University, UGC-SAP and DST-FIST for the facilities. Thanks are due to all friends and fellows for their critical suggestions during experimental set up and manuscript preparation.


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Ghulam Md Ashraf
    • 1
    • 2
  • Naheed Banu
    • 2
  • Ausaf Ahmad
    • 1
    • 2
  • Lamabam Peter Singh
    • 3
  • Rajnish Kumar
    • 1
  1. 1.Amity Institute of Biotechnology (AIB)Amity University Uttar Pradesh (AUUP)LucknowIndia
  2. 2.Department of Biochemistry, Faculty of Life SciencesAligarh Muslim UniversityAligarhIndia
  3. 3.Department of Botany, Faculty of Life SciencesAligarh Muslim UniversityAligarhIndia

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