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The Protein Journal

, Volume 29, Issue 4, pp 265–275 | Cite as

Binding of Al(III)-tetracarboxyphthalocyanine to Hemoglobin and Myoglobin

  • Qiu-Hua Zhou
  • Hong-Mei Zhang
  • Lin Wu
  • Yan-Qing Wang
Article

Abstract

The interactions between Al(III)-tetracarboxyphthalocyanine (AlPc(COOH)4) and hemoglobin (or myoglobin) have been studied. The results showed that AlPc(COOH)4 effectively quenched the intrinsic fluorescence of Hb and Mb via static quenching. The hydrophobic and electrostatic interactions played a major role in stabilizing the AlPc(COOH)4-protein complex. The distance r between donor and acceptor was obtained to be 3.92 and 3.67 nm for AlPc(COOH)4-Hb and AlPc(COOH)4-Mb system, respectively. The effect of AlPc(COOH)4 on the conformation of Hb and Mb was analyzed using UV–vis absorption spectroscopy, circular dichroism spectra, synchronous fluorescence and three-dimensional fluorescence spectra.

Keywords

Al(III)-tetracarboxyphthalocyanine Hemoglobin Myoglobin Fluorescence spectroscopy 

Abbreviation

AlPc(COOH)4

Al(III)-tetracarboxyphthalocyanine

CD

Circular dichroism

FRET

Fluorescence resonance energy transfer

Hb

Hemoglobin

Lys

Lysine

Mb

Myoglobin

RLS

Resonance light scattering spectra

Trp

Tryptophan

Tyr

Tyrosine

Uv-vis

Ultraviolet visible

Notes

Acknowledgments

We gratefully acknowledge financial support of the Natural Science Foundation of Education Department of Jiangsu Province (Grant No. 07KJA18017), the Educational Bureau (Grant No.09KJD150007), the Jiangsu Fundament of “Qilan Project”, and the Scientific Foundation of Yancheng Teachers University.

Supplementary material

10930_2010_9248_MOESM1_ESM.doc (1.1 mb)
Supplementary material 1 (DOC 1162 kb)

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Qiu-Hua Zhou
    • 1
    • 2
  • Hong-Mei Zhang
    • 1
    • 2
  • Lin Wu
    • 1
    • 2
  • Yan-Qing Wang
    • 1
    • 2
  1. 1.Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental ProtectionYanchengChina
  2. 2.Chemistry Department, Institute of Applied Chemistry and Environmental EngineeringYancheng Teachers UniversityYancheng CityPeople’s Republic of China

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